CAJ | 학술논문

制备嗜水气单胞菌 MOM P-ISCOMs 疫苗，使用该疫苗口服和注射免疫欧洲鳗鲡，分别于口服或注射免疫后第14、28 d 分离欧洲鳗鲡的皮肤和肾脏细胞，使用 ELISPOT 技术分析其中抗体分泌细胞 ASC 的数量变化。结果显示：2种免疫方式均能不同程度增加皮肤和肾脏中免疫细胞的数量；其中口服免疫组非特异性免疫细胞数量增加速度较快，注射免疫组特异性免疫细胞数量增加更多。
제비기수기단포균 MOM P-ISCOMs 역묘，사용해역묘구복화주사면역구주만려，분별우구복혹주사면역후제14、28 d 분리구주만려적피부화신장세포，사용 ELISPOT 기술분석기중항체분비세포 ASC 적수량변화。결과현시：2충면역방식균능불동정도증가피부화신장중면역세포적수량；기중구복면역조비특이성면역세포수량증가속도교쾌，주사면역조특이성면역세포수량증가경다。
Aeromonas hydrophila MOM P-ISCOMs vaccine was prepared , and the vaccine was given to European eel by oral or injection .The European eel skin and kidney cells were separated and isolated after immunization 14 and 28 days respectively ,in which the antibody secreting cells (ASC) ,were analyzed by ELISPOT technique .The results showed that the two immune ways could affect the number of immune cells in skin and kidneys in different amount of non-specific immune cells increased rapidly in oral immunization group , and specific immune cells increased more obviously in injection group .