CAJ | 학술논문

以三明显性核不育为载体，以75-1-127为 Pi-9基因的供体亲本，利用分子标记辅助方法通过连续回交把抗稻瘟病基因 Pi-9导入受体亲本双抗明占中。对 BC4 F3代可育株进行筛选，获得含有纯合 Pi-9基因株系HP304。通过水稻 DNA 指纹图谱鉴定，其遗传背景与双抗明占一致，主要农艺性状与双抗明占没有显著差异；田间自然诱发稻瘟病抗性明显优于双抗明占。实践证明，利用三明显性核不育水稻转导抗稻瘟病 Pi -9基因具有可行性。
이삼명현성핵불육위재체，이75-1-127위 Pi-9기인적공체친본，이용분자표기보조방법통과련속회교파항도온병기인 Pi-9도입수체친본쌍항명점중。대 BC4 F3대가육주진행사선，획득함유순합 Pi-9기인주계HP304。통과수도 DNA 지문도보감정，기유전배경여쌍항명점일치，주요농예성상여쌍항명점몰유현저차이；전간자연유발도온병항성명현우우쌍항명점。실천증명，이용삼명현성핵불육수도전도항도온병 Pi -9기인구유가행성。
Transduction of rice blast resistance gene Pi-9 for rice was studied with Sanming dominant genic male sterile rice as the vector and the rice blast resistance 75-1-127 with Pi-9 gene as the donor . Pi-9 gene was introduced into the rice restore Shuangkangmingzhan by successive backcrosses with supporting molecular markers . The results indicated that the pure lines of HP304 with Pi-9 gene similar to Shuangkangmingzhan had been selected from the fertile plants of BC4 F3 .The basic genetic background of HP304 had been proved to be the same to that of Shuangkangmingzhan . The main agronomic traits of HP304 had no significant difference with Shuangkangmingzhan .And the resistance identification in field showed that the resistant level of HP304 was significantly higher than that of Shuangkangmingzhan .The above demonstrated that it was available to transfer Pi-9 gene into rice using Sanming dominant geneic male sterile as the vector .