福建农业学报
福建農業學報
복건농업학보
FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
2013年
9期
833-843
,共11页
芽孢杆菌%系统发育%平均核苷酸同源性%Jspecies
芽孢桿菌%繫統髮育%平均覈苷痠同源性%Jspecies
아포간균%계통발육%평균핵감산동원성%Jspecies
Bacillus%phylogeny%average nucleotide identity%Jspecies
采用 Jspecies 软件分析芽孢杆菌全基因组之间平均核苷酸同源性（ANI）的特征性。结果表明，芽孢杆菌属间、种间及亚种间两两菌株间 ANI 与其分类地位相关，具有明显属种特异性，其中芽孢杆菌科不同属之间的ANI 值分布于50％～65％；芽孢杆菌种间 ANI 值均低于95％，分布范围为65％～90％，加权平均数为70.12％，其中90％的 ANI 值低于70％；芽孢杆菌亚种间 ANI 值分布主要范围为90％～96％，占90％。因此，芽胞杆菌属间的ANI 鉴定标准建议定为50％～65％，芽胞杆菌种间的 ANI 鉴定标准建议定为65％～90％，芽胞杆菌亚种间的 ANI鉴定标准建议定为90％～96％。同时，基因组中的四核苷酸（Tetra）回归系数与 ANI 值具有相关性，表现明显属种特征性，回归拟合显示两者呈现一元二次方程关系，y ＝271-573.58 x ＋399.65 x2（R2＝0.9812），同时高于70％的ANI 值与相应的 Tetra 回归系数呈线性正相关，方程式为 y ＝178.58x -81.521（R2＝0.8767）。
採用 Jspecies 軟件分析芽孢桿菌全基因組之間平均覈苷痠同源性（ANI）的特徵性。結果錶明，芽孢桿菌屬間、種間及亞種間兩兩菌株間 ANI 與其分類地位相關，具有明顯屬種特異性，其中芽孢桿菌科不同屬之間的ANI 值分佈于50％～65％；芽孢桿菌種間 ANI 值均低于95％，分佈範圍為65％～90％，加權平均數為70.12％，其中90％的 ANI 值低于70％；芽孢桿菌亞種間 ANI 值分佈主要範圍為90％～96％，佔90％。因此，芽胞桿菌屬間的ANI 鑒定標準建議定為50％～65％，芽胞桿菌種間的 ANI 鑒定標準建議定為65％～90％，芽胞桿菌亞種間的 ANI鑒定標準建議定為90％～96％。同時，基因組中的四覈苷痠（Tetra）迴歸繫數與 ANI 值具有相關性，錶現明顯屬種特徵性，迴歸擬閤顯示兩者呈現一元二次方程關繫，y ＝271-573.58 x ＋399.65 x2（R2＝0.9812），同時高于70％的ANI 值與相應的 Tetra 迴歸繫數呈線性正相關，方程式為 y ＝178.58x -81.521（R2＝0.8767）。
채용 Jspecies 연건분석아포간균전기인조지간평균핵감산동원성（ANI）적특정성。결과표명，아포간균속간、충간급아충간량량균주간 ANI 여기분류지위상관，구유명현속충특이성，기중아포간균과불동속지간적ANI 치분포우50％～65％；아포간균충간 ANI 치균저우95％，분포범위위65％～90％，가권평균수위70.12％，기중90％적 ANI 치저우70％；아포간균아충간 ANI 치분포주요범위위90％～96％，점90％。인차，아포간균속간적ANI 감정표준건의정위50％～65％，아포간균충간적 ANI 감정표준건의정위65％～90％，아포간균아충간적 ANI감정표준건의정위90％～96％。동시，기인조중적사핵감산（Tetra）회귀계수여 ANI 치구유상관성，표현명현속충특정성，회귀의합현시량자정현일원이차방정관계，y ＝271-573.58 x ＋399.65 x2（R2＝0.9812），동시고우70％적ANI 치여상응적 Tetra 회귀계수정선성정상관，방정식위 y ＝178.58x -81.521（R2＝0.8767）。
The characteristic of average nucleotide identity (ANI) based on the whole genomes from Bacillus species in Bacillus - like genus was analyzed using the soft of Jspecies . The results showed that there have a species specificity based on the ANI distribution and which correlate with the relationship of taxonomic and evolutionary affinities .The ANI values computed from different Bacillus - like genus were below a 65% identity or above a 50%different in orthologous genes identity .The ANI values from different Bacillus species were below 94% with a wide range from 63.77 to 93.81 and the most of them ranging from 64.77% to 68.4% with the weighted average of 70.12% .The ANI value between subspecies was mainly ranged from 90% to 96% .Our results suggest that the ANI criterion of the identification for genus ,species and subspecies within Bacillus are 50% - 65% , 65% - 90%and 90% - 96% , respectively . Furthermore , Tetra regression index was correlated with the ANI value and displayed the species specificity . Regression fitting analysis showed that the equation of y = 271 - 573.56x +399.65 x2 with the correlation coefficient of 0.981 2 occurred between the Tetra and the ANI values above a identity of 70 were positive correlated with Tetra regression index with the equation of y = 178.58 x - 81.521 and the correlation coefficient of 0.876 7 .
