CAJ | 학술논문

利用实验室分离并保藏的伞枝犁头霉菌株D8，对其液体摇瓶发酵产β-葡萄糖苷酶工艺进行了研究.为了进一步提高该菌株的酶活，对产酶发酵培养过程的动态变化进行研究，采用单因素试验对菌株D8的液态摇瓶发酵培养基和发酵条件进行了优化.其培养基适宜的碳源为玉米粉5％、氮源为尿素0．5％；适宜添加的无机盐为氯化钙、硫酸镁、磷酸氢二钾、硫酸亚铁均为适宜添加无机盐；菌株D8液体摇瓶发酵优化条件为：250 mL三角瓶装料量120 mL液体培养基，适宜初始发酵pH为3．0，发酵温度为25℃，发酵周期8 d，β-葡萄糖苷酶酶活力达到168．7 U/mL .
이용실험실분리병보장적산지리두매균주D8，대기액체요병발효산β-포도당감매공예진행료연구.위료진일보제고해균주적매활，대산매발효배양과정적동태변화진행연구，채용단인소시험대균주D8적액태요병발효배양기화발효조건진행료우화.기배양기괄의적탄원위옥미분5％、담원위뇨소0．5％；괄의첨가적무궤염위록화개、류산미、린산경이갑、류산아철균위괄의첨가무궤염；균주D8액체요병발효우화조건위：250 mL삼각병장료량120 mL액체배양기，괄의초시발효pH위3．0，발효온도위25℃，발효주기8 d，β-포도당감매매활력체도168．7 U/mL .
The fermentation technology of β-glucosidase by Absidia corymbifera in lab was studied. In order to improve the enzyme activity of the strain,the dynamic changes of the enzyme production fermentation process was researched. Compositions of medium and fermentation conditions of the D8 strain were optimized by single factor test. The optimum medium compositions:corn starch 5%;the urea 0.5%;added inorganic salt CaCl2,MgSO4·7H2O, KH2PO4,FeSO4·7H2O;medium volume 120 mL/250 mL,initial pH 3.0,the best temperature 25℃,the time course 8 d. In this way,it could produceβ-glucosidase activity 168.7 U/mL in 7 d.