CAJ | 학술논문

为保护资源和实现栽培，以根茎段为材料，采用试管苗培养的方法，对汉城细辛进行了试管苗培养、试管苗繁殖培养、微型根茎扦插及试管苗移栽和定植的研究.结果证明：1/2MS+蔗糖12 g/L+ABT2号1.2 mg/L+IBA0.4 mg/L是试管苗培养和试管苗根茎培养的适宜培养基；1/2MS+蔗糖10 g/L+ABT2号1.2 mg/L+IBA0.5 mg/L+ IAA0.1 mg/L是试管苗根茎的繁殖培养适宜培养基.微型根茎段成活率为97.0%；试管苗移栽成活率为98.25%；定植成活率为97.8%.定植后保持了野生汉城细辛的植物学性状.
위보호자원화실현재배，이근경단위재료，채용시관묘배양적방법，대한성세신진행료시관묘배양、시관묘번식배양、미형근경천삽급시관묘이재화정식적연구.결과증명：1/2MS+자당12 g/L+ABT2호1.2 mg/L+IBA0.4 mg/L시시관묘배양화시관묘근경배양적괄의배양기；1/2MS+자당10 g/L+ABT2호1.2 mg/L+IBA0.5 mg/L+ IAA0.1 mg/L시시관묘근경적번식배양괄의배양기.미형근경단성활솔위97.0%；시관묘이재성활솔위98.25%；정식성활솔위97.8%.정식후보지료야생한성세신적식물학성상.
In ord er to preserve the wild resources and realize cultivation,with the method of tissue culture,the rootstocks of Asarum sieboidii var seoulense Nakai were used as materials to research. The study consists of the cultivation of tube seedlings,propagation of tube seedlings,cuttings for miniature rhizome segments,transplanting and colonization of tube seedlings. The results demonstrated that 1/2MS+ sucrose 12 g/L+ABTⅡ1.2 mg/L+ IBA 0.4 mg/L was the ideal medium for cultivation of tube seedlings,and 1/2MS+ sucrose 10 g/L+ABTⅡ1.2 mg/L+IBA0.5 mg/L+IAA0.1 mg/L was the ideal medium for propagation of tube seedlings. The survival rate of miniature rhizome segments was 97.0%. The survival rate of transplanted tube seedlings was 98.25% and colonization survival rate was 97.8%. The colonization of plantlets maintained all biological traits of wild plant.