浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2013年
10期
787-789,859
,共4页
李海峰%王慧%夏哲智%赵彦婷
李海峰%王慧%夏哲智%趙彥婷
리해봉%왕혜%하철지%조언정
大鼠%癫痫%石菖蒲水煎剂%认知功能
大鼠%癲癇%石菖蒲水煎劑%認知功能
대서%전간%석창포수전제%인지공능
rats%epilepsy%rhizoma acori tatarinowii%cognitive
目的：：探讨石菖蒲水煎剂对癫痫大鼠脑部神经发生的影响和对癫痫认知障碍的治疗作用。方法：SD大鼠30只，随机分为正常对照组、癫痫模型组和石菖蒲灌胃组，每组10只。癫痫模型组和石菖蒲灌胃组采用印防己碱（PTX）建立癫痫动物模型，采用5-溴脱氧尿苷嘧啶（5-bromo-2-deoxyuridine，BrdU）腹腔注射标记内源性神经干细胞（NSC）。正常对照组、癫痫模型组予生理盐水灌胃，石菖蒲灌胃组予石菖蒲水煎剂10mg/（kg·d）灌胃，共30天。治疗前后进行鼠癫痫发作Racine评级、皮层脑电图检测、脑内BrdU阳性细胞免疫组化检测和Morris水迷宫检测。结果：Morris水迷宫检测显示，癫痫模型组逃避潜伏时间长于石菖蒲灌胃组和正常对照组（P<0.05）；癫痫模型组目标象限内的游泳时间占整个游泳时间的百分率明显低于石菖蒲灌胃组和正常对照组（P<0.05）。石菖蒲灌胃组大鼠芋~吁级癫痫发作频率、皮层脑电图频率、波幅降低、Brdu阳性细胞数升高，与癫痫模型组比较，差异有统计学意义（P<0.05）。结论：大鼠发生癫痫后，启动自我保护机制，石菖蒲干预后明显增强了保护机制。
目的：：探討石菖蒲水煎劑對癲癇大鼠腦部神經髮生的影響和對癲癇認知障礙的治療作用。方法：SD大鼠30隻，隨機分為正常對照組、癲癇模型組和石菖蒲灌胃組，每組10隻。癲癇模型組和石菖蒲灌胃組採用印防己堿（PTX）建立癲癇動物模型，採用5-溴脫氧尿苷嘧啶（5-bromo-2-deoxyuridine，BrdU）腹腔註射標記內源性神經榦細胞（NSC）。正常對照組、癲癇模型組予生理鹽水灌胃，石菖蒲灌胃組予石菖蒲水煎劑10mg/（kg·d）灌胃，共30天。治療前後進行鼠癲癇髮作Racine評級、皮層腦電圖檢測、腦內BrdU暘性細胞免疫組化檢測和Morris水迷宮檢測。結果：Morris水迷宮檢測顯示，癲癇模型組逃避潛伏時間長于石菖蒲灌胃組和正常對照組（P<0.05）；癲癇模型組目標象限內的遊泳時間佔整箇遊泳時間的百分率明顯低于石菖蒲灌胃組和正常對照組（P<0.05）。石菖蒲灌胃組大鼠芋~籲級癲癇髮作頻率、皮層腦電圖頻率、波幅降低、Brdu暘性細胞數升高，與癲癇模型組比較，差異有統計學意義（P<0.05）。結論：大鼠髮生癲癇後，啟動自我保護機製，石菖蒲榦預後明顯增彊瞭保護機製。
목적：：탐토석창포수전제대전간대서뇌부신경발생적영향화대전간인지장애적치료작용。방법：SD대서30지，수궤분위정상대조조、전간모형조화석창포관위조，매조10지。전간모형조화석창포관위조채용인방기감（PTX）건립전간동물모형，채용5-추탈양뇨감밀정（5-bromo-2-deoxyuridine，BrdU）복강주사표기내원성신경간세포（NSC）。정상대조조、전간모형조여생리염수관위，석창포관위조여석창포수전제10mg/（kg·d）관위，공30천。치료전후진행서전간발작Racine평급、피층뇌전도검측、뇌내BrdU양성세포면역조화검측화Morris수미궁검측。결과：Morris수미궁검측현시，전간모형조도피잠복시간장우석창포관위조화정상대조조（P<0.05）；전간모형조목표상한내적유영시간점정개유영시간적백분솔명현저우석창포관위조화정상대조조（P<0.05）。석창포관위조대서우~우급전간발작빈솔、피층뇌전도빈솔、파폭강저、Brdu양성세포수승고，여전간모형조비교，차이유통계학의의（P<0.05）。결론：대서발생전간후，계동자아보호궤제，석창포간예후명현증강료보호궤제。
Objective:To explore the effect of the water decoction of rhizoma acori tatarinowii on brain neurogenesis and cognitive handicap of epilepsy in rats. Methods:Thirty SD rats were randomly divided into 3 groups,including control group,model group,and intervention group. Epilepsy model was established by picrotoxin in the model and intervention groups. Rats in the intervention group was given the water decoction of rhizoma acori tatarinowii of 10 mg/(kg·d) for 30d. Saline was given in the same way as described in the intervention group to the control and model groups. Studies on SVZ and DG region of BrdU positive cells in rat brain and immunohistochemical detec-tion and Morris water maze experiment were carried out. Results:Morris water maze results showed that escape la tency time of the model group was longer than that of the intervention group and the control group(P<0.05). In spatial probe test,the percentage of the swimming time of the model group was significantly lower than that of the intervention and control groups(P<0.05). Cortical EEG results revealed that the Rhizoma acori tatarinowii interven tion improved the cerebral cortex stimulation threshold(P<0.05). Brdu+ cell number of the intervention group signif icantly increased as compared with that of the model group(P<0.05). Conclusion:Epilepsy rats start the self-pro-tection mechanism,and the rhizoma acori tatarinowii intervention can obviously enhance the protection mechanism.
