中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2013年
10期
778-782
,共5页
百草枯%水杨酸钠%肺损伤
百草枯%水楊痠鈉%肺損傷
백초고%수양산납%폐손상
paraquat%sodium salicylate%lung injury
目的：探讨预混水杨酸钠（NaSAL）对急性百草枯（PQ）中毒大鼠生存率及肺损伤的影响。方法 Wistar大鼠59只随机分为正常对照组、NaSAL对照组、2组PQ组和2组PQ+NaSAL预混组。正常对照组（n＝6）采用生理盐水（NS）腹腔注射；NaSAL对照组（n＝6）予腹腔NaSAL（300mg/kg）；2组PQ组用PQ[25mg/kg（n＝11）或35mg/kg（n＝12）]腹腔注射一次性染毒；2组预混组（n＝12）：分别一次性给予PQ（25mg/kg）和NaSAL（200mg/kg）预混溶液，以及PQ（35mg/kg）和NaSAL（300mg/kg）预混溶液。观察各组28d生存率及肺病理学改变。结果 PQ25mg/kg组与PQ35mg/kg组生存率分别为36%和50%，2组预混组的生存率分别为33%和50%，PQ组与预混组各组间28d生存率差异无统计学意义（P＞0.05）。病理学研究显示PQ组急性期肺病变主要为弥漫性肺泡炎性损伤，表现为肺泡及肺泡间隔炎性细胞浸润，肺泡腔内出血、渗出，后期主要表现为纤维组织增生沉积。预混组的病理学改变与PQ组相比无明显改善。结论从28d生存率和病理学角度观察，预混NaSAL对急性PQ中毒大鼠的肺损伤无保护作用。PQ和NaSAL混合后对PQ的毒性无明显影响。
目的：探討預混水楊痠鈉（NaSAL）對急性百草枯（PQ）中毒大鼠生存率及肺損傷的影響。方法 Wistar大鼠59隻隨機分為正常對照組、NaSAL對照組、2組PQ組和2組PQ+NaSAL預混組。正常對照組（n＝6）採用生理鹽水（NS）腹腔註射；NaSAL對照組（n＝6）予腹腔NaSAL（300mg/kg）；2組PQ組用PQ[25mg/kg（n＝11）或35mg/kg（n＝12）]腹腔註射一次性染毒；2組預混組（n＝12）：分彆一次性給予PQ（25mg/kg）和NaSAL（200mg/kg）預混溶液，以及PQ（35mg/kg）和NaSAL（300mg/kg）預混溶液。觀察各組28d生存率及肺病理學改變。結果 PQ25mg/kg組與PQ35mg/kg組生存率分彆為36%和50%，2組預混組的生存率分彆為33%和50%，PQ組與預混組各組間28d生存率差異無統計學意義（P＞0.05）。病理學研究顯示PQ組急性期肺病變主要為瀰漫性肺泡炎性損傷，錶現為肺泡及肺泡間隔炎性細胞浸潤，肺泡腔內齣血、滲齣，後期主要錶現為纖維組織增生沉積。預混組的病理學改變與PQ組相比無明顯改善。結論從28d生存率和病理學角度觀察，預混NaSAL對急性PQ中毒大鼠的肺損傷無保護作用。PQ和NaSAL混閤後對PQ的毒性無明顯影響。
목적：탐토예혼수양산납（NaSAL）대급성백초고（PQ）중독대서생존솔급폐손상적영향。방법 Wistar대서59지수궤분위정상대조조、NaSAL대조조、2조PQ조화2조PQ+NaSAL예혼조。정상대조조（n＝6）채용생리염수（NS）복강주사；NaSAL대조조（n＝6）여복강NaSAL（300mg/kg）；2조PQ조용PQ[25mg/kg（n＝11）혹35mg/kg（n＝12）]복강주사일차성염독；2조예혼조（n＝12）：분별일차성급여PQ（25mg/kg）화NaSAL（200mg/kg）예혼용액，이급PQ（35mg/kg）화NaSAL（300mg/kg）예혼용액。관찰각조28d생존솔급폐병이학개변。결과 PQ25mg/kg조여PQ35mg/kg조생존솔분별위36%화50%，2조예혼조적생존솔분별위33%화50%，PQ조여예혼조각조간28d생존솔차이무통계학의의（P＞0.05）。병이학연구현시PQ조급성기폐병변주요위미만성폐포염성손상，표현위폐포급폐포간격염성세포침윤，폐포강내출혈、삼출，후기주요표현위섬유조직증생침적。예혼조적병이학개변여PQ조상비무명현개선。결론종28d생존솔화병이학각도관찰，예혼NaSAL대급성PQ중독대서적폐손상무보호작용。PQ화NaSAL혼합후대PQ적독성무명현영향。
Objective To investigate the effect of premixed sodium salicylate(NaSAL) solution on the survival and lung injury in rats after acute paraquat(PQ) intoxication. Methods A total of 59 Wistar rats were randomly divided into 6 groups as follows:normal control group(normal saline, n=6), NaSAL control group(n=6), 2 PQ groups and 2 pre-mixed PQ+NaSAL groups. In PQ groups, rats were injected intraperitoneally with PQ of 25mg/kg (n=11) or 35mg/kg(n=12), and then, with 0.9%NaCl at 2h later. Pre-mixed PQ+NaSAL groups were divided into pre-mixed 25mg/kg PQ and 200mg/kg NaSAL, (n=12), and pre-mixed 35mg/kg PQ and 300mg/kg NaSAL(n=12). In NaSAL control group, rats were administered with 0.9%NaCl solution, and then dosed with 300mg/kg NaSAL at 2h later. All groups were observed daily for survival rate and poisoning symptoms over a period of at least 28d. Lung tissues were collected from rats died in the acute stage and those survived over 28d, and then observed with HE and Masson staining for pathological changes. Results The survival rates were 36%and 50%respectively in the 25mg/kg PQ group and 35mg/kg PQ group, and 33% and 50% respectively in the two premixed groups respectively. No significant difference was observed in the 28-day survival rates between PQ groups and pre-mixed PQ+NaSAL groups(P>0.05). Pathological observation showed that the rats exposed to PQ showed apparent lung changes at day 4 (acute stage), including inflammatory cells infiltration into alveoli and alveolar septum, alveolar hemorrhage and effusion at the acute stage, and proliferation and accumulation of fibrotic tissues at the late stage. There was no significant difference in pathological changes between the PQ groups and pre-mixed PQ+NaSAL groups. Conclusions Pre-mixed PQ with NaSAL shows no significant protective effect on rat lung injury induced by PQ intoxication. A pre-mixed PQ and NaSAL solution doesn’t counteract the toxic effect of PQ.
