CAJ | 학술논문

目的：：研究复方大果木姜子软胶囊对小鼠骨髓癌细胞(Sp2/0)增殖抑制作用。方法：取Sp2/0细胞,复苏后以含10%新生牛血清的RPMI1640培养液培养,在37℃、5%CO2的饱和湿环境的培养箱中培养,用细胞排染法测定药物作用,同时设药物组,阳性组,空白组,其中药物组加复方大果木姜子软胶囊,阳性组加喜树碱。生长曲线法测定这三组对sp2/0细胞的增殖抑制作用,MTT法测定三组成分对sp2/0细胞的杀伤作用。结果：实验证明复方大果木姜子软胶囊对Sp2/0细胞的ED50为11.36μg·ml-1,其IC50值为7.725μg·ml-1。结论：一定浓度的实验药物对小鼠骨髓癌细胞Sp2/0的增殖具有明显的抑制作用。但与喜树碱相比,效果还是有一定差距。如果进一步筛选复方中主要单味有效成分,再组合研究其药效,一定可以进一步提高其对Sp2/0细胞的增殖抑制作用,同时减少药物用量。
목적：：연구복방대과목강자연효낭대소서골수암세포(Sp2/0)증식억제작용。방법：취Sp2/0세포,복소후이함10%신생우혈청적RPMI1640배양액배양,재37℃、5%CO2적포화습배경적배양상중배양,용세포배염법측정약물작용,동시설약물조,양성조,공백조,기중약물조가복방대과목강자연효낭,양성조가희수감。생장곡선법측정저삼조대sp2/0세포적증식억제작용,MTT법측정삼조성분대sp2/0세포적살상작용。결과：실험증명복방대과목강자연효낭대Sp2/0세포적ED50위11.36μg·ml-1,기IC50치위7.725μg·ml-1。결론：일정농도적실험약물대소서골수암세포Sp2/0적증식구유명현적억제작용。단여희수감상비,효과환시유일정차거。여과진일보사선복방중주요단미유효성분,재조합연구기약효,일정가이진일보제고기대Sp2/0세포적증식억제작용,동시감소약물용량。
Objective:To study the effect of Cinna momum migao H.W.Li soft capsule inhibition on proliferation of mouse cellline SP2/0 in vitro. Methods:When mouse cellline SP2/0 was resuscitated,they were cultured in culture medium containing 10%newborn calf serum RPMI1640 at 37℃and 5%CO2 saturated moist environment.celldye exclusion method was used to determine the drμg action.At the same time,we set the drμg groups,the positive group and blank group.Cinna momum migao H.W.Li soft capsule was added to drμg group,and camptothecin to positive group.Growth curve method was used to determine the three groups inhibition on the sp2/0 cellproliferation,and MTT method to determine the kil ing effect on the sp2/0 cell. Results:The experiments results proved that the ED50 of Cinna momum migao H.W.Li soft capsule on Sp2/0 cells was 11.36μg·ml-1, its IC50 value was 7.725μg·ml-1.Conclusion:A certain concentration of experimental drμgs have obvious inhibitory effect for SP2/0.But compared with camptothecin,the effect is stil have a big gap.If we further screened the single main effective components in the compound and combined with the efficacy study,we can improve the proliferation inhibition on Sp2/0 cell,as wel as reduce drμg dosage.