中国临床新医学
中國臨床新醫學
중국림상신의학
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
2013年
10期
942-944
,共3页
覃桂芳%李友琼%卢秋维%罗焰芳%夏莉莉
覃桂芳%李友瓊%盧鞦維%囉燄芳%夏莉莉
담계방%리우경%로추유%라염방%하리리
EB病毒Rta-IgG%定量检测%鼻咽癌%诊断
EB病毒Rta-IgG%定量檢測%鼻嚥癌%診斷
EB병독Rta-IgG%정량검측%비인암%진단
EBV Rta-IgG%Quantitative detection%Nasopharyngeal carcinoma ( NPC)%Diagnosis
目的：探讨EB病毒Rta蛋白IgG抗体在鼻咽癌诊断中的临床价值。方法采用间接ELISA法检测612例临床样本中EB病毒Rta-IgG含量，同时对试剂盒的分析性能进行评估。结果267例鼻咽癌样本的EB病毒Rta-IgG抗体浓度为（77.64±3.205）U/ml，检测灵敏度为94.7％（214/226），特异度为86.3％（333/386），正确率为89.4％（547/612）；255例健康体检血样抗体浓度为（8.31±0.745）U/ml；20例肝癌样本抗体浓度为（7.14±1.111） U/ml；30例肝炎样本抗体浓度为（7.40±0.581） U/ml；20例自身免疫病样本抗体浓度为（13.97±6.943）U/ml；20例鼻咽息肉样本抗体浓度为（12.58±6.197）U/ml。各组EB病毒Rta-IgG含量比较差异有统计学意义（ P＜0.05）。结论 EB病毒Rta-IgG定量检测试剂盒用于鼻咽癌诊断具有较高的临床价值，为进一步研究鼻咽癌患者治疗效果与患者血清中EB病毒Rta-IgG的含量的关系奠定了基础。
目的：探討EB病毒Rta蛋白IgG抗體在鼻嚥癌診斷中的臨床價值。方法採用間接ELISA法檢測612例臨床樣本中EB病毒Rta-IgG含量，同時對試劑盒的分析性能進行評估。結果267例鼻嚥癌樣本的EB病毒Rta-IgG抗體濃度為（77.64±3.205）U/ml，檢測靈敏度為94.7％（214/226），特異度為86.3％（333/386），正確率為89.4％（547/612）；255例健康體檢血樣抗體濃度為（8.31±0.745）U/ml；20例肝癌樣本抗體濃度為（7.14±1.111） U/ml；30例肝炎樣本抗體濃度為（7.40±0.581） U/ml；20例自身免疫病樣本抗體濃度為（13.97±6.943）U/ml；20例鼻嚥息肉樣本抗體濃度為（12.58±6.197）U/ml。各組EB病毒Rta-IgG含量比較差異有統計學意義（ P＜0.05）。結論 EB病毒Rta-IgG定量檢測試劑盒用于鼻嚥癌診斷具有較高的臨床價值，為進一步研究鼻嚥癌患者治療效果與患者血清中EB病毒Rta-IgG的含量的關繫奠定瞭基礎。
목적：탐토EB병독Rta단백IgG항체재비인암진단중적림상개치。방법채용간접ELISA법검측612례림상양본중EB병독Rta-IgG함량，동시대시제합적분석성능진행평고。결과267례비인암양본적EB병독Rta-IgG항체농도위（77.64±3.205）U/ml，검측령민도위94.7％（214/226），특이도위86.3％（333/386），정학솔위89.4％（547/612）；255례건강체검혈양항체농도위（8.31±0.745）U/ml；20례간암양본항체농도위（7.14±1.111） U/ml；30례간염양본항체농도위（7.40±0.581） U/ml；20례자신면역병양본항체농도위（13.97±6.943）U/ml；20례비인식육양본항체농도위（12.58±6.197）U/ml。각조EB병독Rta-IgG함량비교차이유통계학의의（ P＜0.05）。결론 EB병독Rta-IgG정량검측시제합용우비인암진단구유교고적림상개치，위진일보연구비인암환자치료효과여환자혈청중EB병독Rta-IgG적함량적관계전정료기출。
Objective To investigate the clinical value of Epstein-Barr virus ( EBV) Rta/IgG in the diagno-sis of nasopharyngeal carcinoma ( NPC) .Methods Sera from 612 clinical samples were measured for the Rta-IgG by an indirect enzyme-linked immunosorbent assay .In the present study , we also evaluated the performance of the Rta-IgG ELISA kits.Results The mean value of Rta-IgG concentration in 267 NPC patients was (77.64 ±3.205)U/ml with a sensitivity of 94.7%( 214/226 ) and specificity of 86.3%( 333/386 ) and accuracy of 89.4%( 547/612 ) . The mean value of Rta-IgG concentration in 255 healthy volunteers ,20 hepatocellular carcinoma patients ,30 hepatitis patients,20 patients with autoimmune disease and 20 patients with nasopharyngeal polyp were (8.31 ±0.745)U/ml, (7.14 ±1.111)U/ml, (7.40 ±0.581)U/ml, (13.97 ±6.943)U/ml and (12.58 ±6.197)U/ml, respectively. There were significant difference between NPC group and other groups .Conclusion Our results suggest that the EBV Rta/IgG ELISA kit is suitable for the clinical diagnosis of NPC .It also provides a basis for further study on the rela-tionship between the therapeutic effect and serum EBV Rta-IgG content in NPC patients .
