CAJ | 학술논문

目的：探讨白花蛇舌草乙醇提取物对BALB/c小鼠CT-26结肠癌细胞株增殖的抑制作用．方法采用甲基噻唑蓝（MTT）法测定不同浓度白花蛇舌草乙醇提取物对CT-26细胞株的生长抑制作用，计算半数抑制浓度（IC50）．结果在同一浓度下，白花蛇舌草乙醇提取物对CT-26细胞的抑制作用随着作用时间的延长而增强，0.08 mg/mL的白花蛇舌草乙醇提取物作用CT-26细胞24 h、48 h、72 h，细胞抑制率分别为（16.67±9.35）%，（34.66±9.23）%，（40.07±9.16）%．白花蛇舌草乙醇提取物作用24 h、48 h、72 h 的 IC50值分别为0.315，0.155，0.115．在同一作用时间内，白花蛇舌草乙醇提取物对CT-26细胞的抑制率随着药物浓度的增加而增加，在72 h内不同浓度药物（0.06 mg/mL，0.08 mg/mL，0.10 mg/mL，0.12 mg/mL，0.14 mg/mL，0.16 mg/mL，0.18 mg/mL，0.20 mg/mL）作用下，细胞抑制率分别为（35.46±3.59）%，（40.07±9.16）%，（40.77±6.92）%，（52.81±1.87）%，（54.22±2.35）%，（68.72±3.71）%，（70.04±8.03）%，（71.84±3.12）%．结论白花蛇舌草乙醇提取物可以抑制BALB/c小鼠CT-26结肠癌细胞株的增殖，其对CT-26细胞增殖的抑制作用呈现时效和量效的关系．
목적：탐토백화사설초을순제취물대BALB/c소서CT-26결장암세포주증식적억제작용．방법채용갑기새서람（MTT）법측정불동농도백화사설초을순제취물대CT-26세포주적생장억제작용，계산반수억제농도（IC50）．결과재동일농도하，백화사설초을순제취물대CT-26세포적억제작용수착작용시간적연장이증강，0.08 mg/mL적백화사설초을순제취물작용CT-26세포24 h、48 h、72 h，세포억제솔분별위（16.67±9.35）%，（34.66±9.23）%，（40.07±9.16）%．백화사설초을순제취물작용24 h、48 h、72 h 적 IC50치분별위0.315，0.155，0.115．재동일작용시간내，백화사설초을순제취물대CT-26세포적억제솔수착약물농도적증가이증가，재72 h내불동농도약물（0.06 mg/mL，0.08 mg/mL，0.10 mg/mL，0.12 mg/mL，0.14 mg/mL，0.16 mg/mL，0.18 mg/mL，0.20 mg/mL）작용하，세포억제솔분별위（35.46±3.59）%，（40.07±9.16）%，（40.77±6.92）%，（52.81±1.87）%，（54.22±2.35）%，（68.72±3.71）%，（70.04±8.03）%，（71.84±3.12）%．결론백화사설초을순제취물가이억제BALB/c소서CT-26결장암세포주적증식，기대CT-26세포증식적억제작용정현시효화량효적관계．
Objective To explore the inhibitory effect of the ethanol extract of Oldenlandia diffusa on the proliferation of CT-26 colon cancer cells which come from BALB/c mice. Method We determined the inhibitory effect of different concentrations of ethanol extract of Oldenlandia diffusa on CT-26 cells' proliferation by using methyl thiazolyl tetrazolium (MTT), and calculated the 50% inhibiting concentration (IC50) . Results As to the same concentration, the inhibitory effect of the ethanol extract of Oldenlandia diffusa on CT-26 cells was increased with time, for exsample:after treated with 0.08 mg/mL of ethanol extract of Oldenlandia diffusa for 24 h, 48 h and 72 h, the inhibitory rates of CT-26 cells were (16.67 ±9.35)%, (34.66 ±9.23)%and (40.07 ±9.16)%, respectively. After treating CT-26 cancer cells for 24 h, 48 h and 72 h, the IC50 values of the ethanol extract of Oldenlandia diffusa were 0.315,0.155 and 0.115 mg/mL, respectively. In the same treatment time, the inhibitory effect of the ethanol extract of Oldenlandia diffusa on CT-26 cells was increased with the increase of concentration:after treatment for 72 h with different concentrations (0.06 mg/mL,0.08 mg/mL,0.10 mg/mL,0.12 mg/mL, 0.14 mg/mL,0.16 mg/mL,0.18 mg/mL and 0.20 mg/mL) of the ethanol extract of Oldenlandia diffusa,the inhibitory rates of CT-26 cells were (35.46 ±3.59)%, (40.07 ±9.16)%, (40.77 ±6.92)%, (52.81 ±1.87)%, (54.22±2.35)%, (68.72±3.71)%, (70.04±8.03)%and (71.84±3.12)%, respectively. Conclusion The ethanol extract of olenlandia diffusa can inhibit the proliferation of CT-26 colon cancer cells from BALB/c mice in a time and dose dependent manner.