CAJ | 학술논문

目的：为建立和评价人类疾病树鼩模型奠定生理生化基础．方法滇西亚种树鼩92只，雌雄各半．禁食12 h后，非麻醉状态下心脏采血0.8～1.0 mL装入灭菌离心管用于分离制备血清并用Olympus AU5400全自动生化分析仪进行生化指标测定．每只树鼩人工挤尿1.5～2.0 mL存于灭菌离心管用Combi-scan500尿液分析仪进行尿液分析测定．用SPSS统计软件对测定的数据统计分析并与人的参考值进行了比较．结果滇西亚种树鼩雌雄间心肌酶与部分肾功能指标测定值中乳酸脱氢酶、α-羟丁酸脱氢酶、肌酐、尿酸、尿比重、酸碱度比较差异有统计学意义（＜0.05），其余的指标差异无统计学意义（＞0.05）．滇西亚种树鼩雌雄合并、雌性和雄性心肌酶与部分肾功能指标测定值与人的参考值比较得出：尿素、尿比重、尿糖、尿胆原、尿胆红素、尿亚硝酸盐均在人的参考值范围内，乳酸脱氢酶、肌酸激酶、α-羟丁酸脱氢酶、肌酸激酶同工酶CKMB均高于人的参考值，肌酐、尿酸和尿酸碱度稍低于人的参考值．白细胞、尿蛋白、酮体、隐血多数与人的参考值一致为阴性，但偶有阳性的，阳性率分别为3.95%、46.1%、39.5%、2.63%．维生素C与人的参考值相反为阳性，但也偶有阴性的，阴性率为6.6%．结论尿素、尿比重、尿糖、尿胆原、尿胆红素、尿亚硝酸盐可直接作为人类疾病树鼩模型的评价指标，其它指标参数可作为判断树鼩正常生理生化的依据．
목적：위건립화평개인류질병수구모형전정생리생화기출．방법전서아충수구92지，자웅각반．금식12 h후，비마취상태하심장채혈0.8～1.0 mL장입멸균리심관용우분리제비혈청병용Olympus AU5400전자동생화분석의진행생화지표측정．매지수구인공제뇨1.5～2.0 mL존우멸균리심관용Combi-scan500뇨액분석의진행뇨액분석측정．용SPSS통계연건대측정적수거통계분석병여인적삼고치진행료비교．결과전서아충수구자웅간심기매여부분신공능지표측정치중유산탈경매、α-간정산탈경매、기항、뇨산、뇨비중、산감도비교차이유통계학의의（＜0.05），기여적지표차이무통계학의의（＞0.05）．전서아충수구자웅합병、자성화웅성심기매여부분신공능지표측정치여인적삼고치비교득출：뇨소、뇨비중、뇨당、뇨담원、뇨담홍소、뇨아초산염균재인적삼고치범위내，유산탈경매、기산격매、α-간정산탈경매、기산격매동공매CKMB균고우인적삼고치，기항、뇨산화뇨산감도초저우인적삼고치．백세포、뇨단백、동체、은혈다수여인적삼고치일치위음성，단우유양성적，양성솔분별위3.95%、46.1%、39.5%、2.63%．유생소C여인적삼고치상반위양성，단야우유음성적，음성솔위6.6%．결론뇨소、뇨비중、뇨당、뇨담원、뇨담홍소、뇨아초산염가직접작위인류질병수구모형적평개지표，기타지표삼수가작위판단수구정상생리생화적의거．
Objective To lay the physiological and biochemical basis for establishing and evaluating tree shrews model of human disease. Methods There were 92 Tupaia belangeri chinensis, in which half of them were male,they were allowed to eat nothing for 12 hours, then we sampled heart blood 0.8~1.0 mL without anesthesia and put blood samples into sterilized centrifuge tube for separation and preparation of serum. Olympus AU5400 automatic biochemistry analyzer was used to measure biochemical indexes. Then 1.5~2.0 mL of urine of each tree shrew was collected and put into sterilized centrifuge tube for measuring renal function by using Combi-scan500 urine analyzer. Fianally SPSS statistics software was used to analyse the measured values, and comapared the measured values with the human reference values. Results There were significant differences in myocardial enzymes and some renal function indexes such as lactic dehydrogenase,α- hydroxybutyric, acid dehydrogenase, creatinine, uric acid, urine specific gravity and pH value between male and female Tupaia belangeri chinensis ( <0.05), and there was no significant difference in the rest of the measured values ( >0.05) . Then determination value of Tupaia belangeri chinensis, male’s and female’s, myocardial enzyme and part of the renal function indexes were compared with the human reference values. Some indexes including urea, urine specific gravity, urine, urobilinogen were in the range of human reference value, while the values of urine bilirubin, urine nitrite. lactate dehydrogenase, creatine kinase, α-hydroxybutyrate dehydrogenase, creatine kinase isoenzyme CKMB were higher than the human reference value. White blood cell, urine protein, ketone, occult blood most of them were negative as the same to human reference value, but sometimes were positive, and the positive rates respectively were 3.95%and 46.1%,39.5%,2.63%. The measured value of Vitamin C was positive that is completely opposite to human reference value, but sometimes is negative, the negative rate was 6.6%. Conclusion Urea, urine specific gravity, urine, urobilinogen, urine bilirubin, urine nitrite can be directly used as the indexes for evaluating tree shrews models of human disease, other indexes can be used as indexes for judgment of the normal physiological and biochemical basis of tree shrews.