CAJ | 학술논문

采用正交试验设计，研究不同基本培养基和激素配比对红心材马尾松胚培养的影响。结果表明，沙子和无菌水培养基上的种子萌发率最高，达到71.6％，苗长得最好。WPM＋6 BA 2 mg/L＋NAA 0.3 mg/L＋KT 0.2 mg/L培养基为最佳增殖培养基，增殖系数最高，达到7.3，芽高度较高、粗壮。以1/2WPM＋ABT1＃0.8 mg/L＋NAA 0.1 mg/L培养基不定芽生根率最高，达到69.7％。
채용정교시험설계，연구불동기본배양기화격소배비대홍심재마미송배배양적영향。결과표명，사자화무균수배양기상적충자맹발솔최고，체도71.6％，묘장득최호。WPM＋6 BA 2 mg/L＋NAA 0.3 mg/L＋KT 0.2 mg/L배양기위최가증식배양기，증식계수최고，체도7.3，아고도교고、조장。이1/2WPM＋ABT1＃0.8 mg/L＋NAA 0.1 mg/L배양기불정아생근솔최고，체도69.7％。
Using orthogonal experimental design ,tissue culture of seeds of red heart-wood of Pi-nus massoniana was researched .The result showed that the germination rate of seeds of Pinus massoniana in the induction medium containing sands and aseptic water was the best with the ger-mination rate of 71 .6% .In the multiplication culture ,the medium of WPM +6-BA 2 mg/L+NAA 0 .3 mg/L+KT 0 .2 mg/L was the best with strong and high buds and the multiplication coeffi-cient of 7 .3 .In rooting culture ,the rooting rate of 1/2WPM+ABT1#0 .8 mg/L+NAA 0 .1 mg/L was the highest ,reaching 69 .7% .This study will lay a foundation for rapid propagation technique system of red heart-wood Pinus massoniana .