CAJ | 학술논문

为进一步提高油料作物亚麻荠的含油量，克隆拟南芥胚珠组织特异型基因的启动子p I NO和拟南芥细胞色素P450 KLUH基因（KLU），以植物表达载体pCAMBIA2301为基础载体，构建组织特异性启动子pINO调控 KLU基因的植物表达载体pCAMBIA2301 pINO KLU ，用根癌农杆菌介导的floral dip法转化亚麻荠。结果表明，农杆菌培养至OD600值为0．8时，用等体积渗入培养基（1/2 MS、5％蔗糖、200μL/L Silwet L 77）重悬菌体转化亚麻荠，50 mg/L卡那霉素检测转基因种子的阳性率为15％，PCR检测初步证明，KLU基因已整合到部分抗性植株的基因组中，转化率为1．8％。
위진일보제고유료작물아마제적함유량，극륭의남개배주조직특이형기인적계동자p I NO화의남개세포색소P450 KLUH기인（KLU），이식물표체재체pCAMBIA2301위기출재체，구건조직특이성계동자pINO조공 KLU기인적식물표체재체pCAMBIA2301 pINO KLU ，용근암농간균개도적floral dip법전화아마제。결과표명，농간균배양지OD600치위0．8시，용등체적삼입배양기（1/2 MS、5％자당、200μL/L Silwet L 77）중현균체전화아마제，50 mg/L잡나매소검측전기인충자적양성솔위15％，PCR검측초보증명，KLU기인이정합도부분항성식주적기인조중，전화솔위1．8％。
For further improving the oil content of an alternative oil crop species Camelina sativ a (L .) Crantz ,we cloned the Arabidopsis ovule organ-specific promoter (pINO) and the Arabi-dopsis cytochrome P450 KLUH gene (KLU) .Next ,the plant expression vector pCAMBIA2301 was digested and fused KLU with pINO to construct the plant express vector pCAMBIA 2301-pI-NO-KLU ,which was introduced into Camelinasativa (L .) Crantz with Agrobacterium-mediated floral dip transformation .The results showed that through using the Agrobacterium pellet to transform Camelina sativa (L .) Crantz ,which was resuspended by the same volume of infiltra-tion medium (1/2MS ,5% sucrose ,200 μL/L Silwet L-77) when Agrobacterium culture was cul tured to OD600 =0 .8 ,positive rate of screening transgenic seeds by Kan (50 mg/L) was 15% .Fur-ther PCR analysis confirmed that KLU gene had been incorporated into the genome of parts of re-sistant plant ,and the transformation efficiency was 1 .8% .