河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2013年
10期
4-7
,共4页
王霞%徐亚维%于晓明
王霞%徐亞維%于曉明
왕하%서아유%우효명
人工合成六倍体小麦%谷氨酰胺合成酶%葡萄糖-6-磷酸脱氢酶%蔗糖磷酸合成酶%基因表达
人工閤成六倍體小麥%穀氨酰胺閤成酶%葡萄糖-6-燐痠脫氫酶%蔗糖燐痠閤成酶%基因錶達
인공합성륙배체소맥%곡안선알합성매%포도당-6-린산탈경매%자당린산합성매%기인표체
synthetic hexaploid wheat%glutaminesynthetase%glucose-6-phosphate dehydroge-nase%sucrose phosphate synthase%gene expression
以人工合成异源六倍体小麦为材料，采用RT PCR技术，对六倍体小麦多倍化初期氮、糖代谢关键酶基因（GS1、GS2、G6PD H 、S PS）的表达情况进行了研究。结果表明，六倍体小麦 GS1基因表达水平较其双亲中值（MPV）显著升高，GS2基因表达水平与其亲本基本一致，而 G6PDH基因和S PS基因表达水平较其M PV出现了不同程度的降低。
以人工閤成異源六倍體小麥為材料，採用RT PCR技術，對六倍體小麥多倍化初期氮、糖代謝關鍵酶基因（GS1、GS2、G6PD H 、S PS）的錶達情況進行瞭研究。結果錶明，六倍體小麥 GS1基因錶達水平較其雙親中值（MPV）顯著升高，GS2基因錶達水平與其親本基本一緻，而 G6PDH基因和S PS基因錶達水平較其M PV齣現瞭不同程度的降低。
이인공합성이원륙배체소맥위재료，채용RT PCR기술，대륙배체소맥다배화초기담、당대사관건매기인（GS1、GS2、G6PD H 、S PS）적표체정황진행료연구。결과표명，륙배체소맥 GS1기인표체수평교기쌍친중치（MPV）현저승고，GS2기인표체수평여기친본기본일치，이 G6PDH기인화S PS기인표체수평교기M PV출현료불동정도적강저。
The synthetic hexaploid wheats were used as material to study the expression of key en-zymes genes (GS1 ,GS2 ,G6PD H and S PS) related to nitrogen and sugar metabolism in the early period of wheat allopolyploidization by RT-PCR technology .The results showed that the GS1 gene expression level of hexaploid wheat was much higher than its MPV ,while the GS2 gene ex-pression level of hexaploid wheat was consistent with its parents .The G6PDH and SPS gene ex-pression levels in the hexaploid wheat were lower than its MPV in different degrees .
