风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2013年
10期
23-28
,共6页
脊柱炎,强直性%肾虚寒湿证%阳和汤%肿瘤坏死因子-α%转化生长因子-β%基质金属蛋白酶-9%RANKL%免疫组化
脊柱炎,彊直性%腎虛寒濕證%暘和湯%腫瘤壞死因子-α%轉化生長因子-β%基質金屬蛋白酶-9%RANKL%免疫組化
척주염,강직성%신허한습증%양화탕%종류배사인자-α%전화생장인자-β%기질금속단백매-9%RANKL%면역조화
spondylitis,ankylosing%kidney deficiency cold dampness syndrome%yanghe decoction%tumor necrosis factor-α%transforming growth factor-β%matrix metalloproteinase-9%RANKL%immunohistochemistry
目的：观察阳和汤加减的调节免疫、抑制关节炎症及骨质破坏的作用，初步从细胞、分子水平分析其部分作用机制。方法：采用蛋白聚糖+弗氏佐剂+寒湿因素病证结合的方法制造AS肾虚寒湿型动物模型。采用免疫组织化学技术SP法检测肿瘤坏死因子-α、基质金属蛋白酶-9以及RANKL、转化生长因子-β在骨组织中的表达情况。结果：①复方组、桂枝组、麻黄组、鹿角片组与模型组比较，差异有统计学意义（P<0.05），中药组均发挥疗效。②复方组、桂枝组与模型组比较，差异有统计学意义（P<0.05），复方组与桂枝组药效突出。③复方组与桂枝组、麻黄组、鹿角片组比较，差异有统计学意义（P<0.05），复方组疗效优于其他中药组。结论：阳和汤能够从多环节、多方面发挥调节免疫、抑制关节炎症及骨质破坏作用，达到良好的治疗效果。
目的：觀察暘和湯加減的調節免疫、抑製關節炎癥及骨質破壞的作用，初步從細胞、分子水平分析其部分作用機製。方法：採用蛋白聚糖+弗氏佐劑+寒濕因素病證結閤的方法製造AS腎虛寒濕型動物模型。採用免疫組織化學技術SP法檢測腫瘤壞死因子-α、基質金屬蛋白酶-9以及RANKL、轉化生長因子-β在骨組織中的錶達情況。結果：①複方組、桂枝組、痳黃組、鹿角片組與模型組比較，差異有統計學意義（P<0.05），中藥組均髮揮療效。②複方組、桂枝組與模型組比較，差異有統計學意義（P<0.05），複方組與桂枝組藥效突齣。③複方組與桂枝組、痳黃組、鹿角片組比較，差異有統計學意義（P<0.05），複方組療效優于其他中藥組。結論：暘和湯能夠從多環節、多方麵髮揮調節免疫、抑製關節炎癥及骨質破壞作用，達到良好的治療效果。
목적：관찰양화탕가감적조절면역、억제관절염증급골질파배적작용，초보종세포、분자수평분석기부분작용궤제。방법：채용단백취당+불씨좌제+한습인소병증결합적방법제조AS신허한습형동물모형。채용면역조직화학기술SP법검측종류배사인자-α、기질금속단백매-9이급RANKL、전화생장인자-β재골조직중적표체정황。결과：①복방조、계지조、마황조、록각편조여모형조비교，차이유통계학의의（P<0.05），중약조균발휘료효。②복방조、계지조여모형조비교，차이유통계학의의（P<0.05），복방조여계지조약효돌출。③복방조여계지조、마황조、록각편조비교，차이유통계학의의（P<0.05），복방조료효우우기타중약조。결론：양화탕능구종다배절、다방면발휘조절면역、억제관절염증급골질파배작용，체도량호적치료효과。
Objective:By analysis the expression of tumor necrosis factor-α,matrix metalloproteinases-9,RANKL and transforming growth factor-β in bone tissues to observe the functions of Yanghe Variant Decoction in regulating immunity,inhibiting arthritis and the destruction of bone,making a preliminary analysis of its mechanism from the cell,molecular level.Methods:Ankylosing spondylitis animal models with asthenia renal cold were made by the combination of proteoglycan,Freund's adjuvant and cold damp factors.Tumor necrosis factor-α,matrix metalloproteinases-9,RANKL and the expression of transforming growth factor-β in bone tissue were detected by immunohistochemistry technique SP.Results:①Comparing the compound group,the guizhi group,the mahuang group,the lujiaopian group with the model group,the difference was statistically signiifcant(P<0.05),and all the Chinese medicine groups had curative effects.②Comparing the compound group,the guizhi group with the model group,the difference was statistically signiifcant(P<0.05),and the compound group and the guizhi group had prominent efifcacy. ③ Comparing the compound group with the guizhi group,the mahuang group and the lujiaopian group,the difference was statistically signiifcant(P<0.05),and efifcacy of the compound group was better than the other traditional Chinese medicine groups.Conclusion:Yanghe decoction could from many aspects regulated immunity and inhibited joint inlfammation and bone destruction,bring about good treatment results.
