大理学院学报
大理學院學報
대이학원학보
JOURNAL OF DALI COLLEGE
2013年
10期
44-46,47
,共4页
杨杨%刘毕琴%张发%廖光辉%杨晓燕
楊楊%劉畢琴%張髮%廖光輝%楊曉燕
양양%류필금%장발%료광휘%양효연
α-淀粉酶产生菌%酶活力%分离%筛选
α-澱粉酶產生菌%酶活力%分離%篩選
α-정분매산생균%매활력%분리%사선
α-amylase producing bacteria%enzymatic activity%separation%screening
目的：从土壤中筛选产α-淀粉酶活力较强的菌株并对其酶学性质进行初步研究。方法：结合选择性分离培养，碘液检测法，酶活力测定和形态学检测等方法从土壤中分离出产酶活力较强的菌株，并对其所产α-淀粉酶的最适反应温度和pH值进行研究。结果：共分离到4株能产α-淀粉酶的菌株，其中菌株4产酶活力最高。该菌株所产α-淀粉酶最适反应温度为50~60℃，最适pH值为6.2。结论：分离到1株产α-淀粉酶酶活力较强的芽孢杆菌，所产α-淀粉酶属于中温淀粉酶，最适pH值为6.2。
目的：從土壤中篩選產α-澱粉酶活力較彊的菌株併對其酶學性質進行初步研究。方法：結閤選擇性分離培養，碘液檢測法，酶活力測定和形態學檢測等方法從土壤中分離齣產酶活力較彊的菌株，併對其所產α-澱粉酶的最適反應溫度和pH值進行研究。結果：共分離到4株能產α-澱粉酶的菌株，其中菌株4產酶活力最高。該菌株所產α-澱粉酶最適反應溫度為50~60℃，最適pH值為6.2。結論：分離到1株產α-澱粉酶酶活力較彊的芽孢桿菌，所產α-澱粉酶屬于中溫澱粉酶，最適pH值為6.2。
목적：종토양중사선산α-정분매활력교강적균주병대기매학성질진행초보연구。방법：결합선택성분리배양，전액검측법，매활력측정화형태학검측등방법종토양중분리출산매활력교강적균주，병대기소산α-정분매적최괄반응온도화pH치진행연구。결과：공분리도4주능산α-정분매적균주，기중균주4산매활력최고。해균주소산α-정분매최괄반응온도위50~60℃，최괄pH치위6.2。결론：분리도1주산α-정분매매활력교강적아포간균，소산α-정분매속우중온정분매，최괄pH치위6.2。
Objective:To choose the strain with higher activity in the production ofα-amylase in the soil and preliminary test its enzy-matic property. Methods: The optimal reaction temperature, optimal pH value in the production of α-amylase was tested after the strains with higher activity were separated with the methods of selective separation, iodine test, morphologic detection and determina-tion of enzyme activity. Results:The fourth strain was highest in activity among four of producingα-amylase strains. Its optimal reac-tion temperature was 50-60℃and optimal pH value was 6.2. Conclusion:A strain which has high activity in producingα-amylase bacillus is separated and itsα-amylase is medium temperature amylase with the optimal pH value of 6.2.
