CAJ | 학술논문

目的：建立稀有白血病融合基因MLL/AFX的检测方法。方法运用逆转录-多重PCR扩增目的条带，脱氧核糖核酸测序分析，并对1例急性部分分化型原始粒细胞白血病（M2）患者进行了方法学验证。结果该患者MLL/AFX融合基因阳性。结论逆转录-多重PCR结合测序的方法能够准确地鉴定罕见白血病融合基因MLL/AFX。该方法是一种灵敏、快速的白血病融合基因检测方法。
목적：건립희유백혈병융합기인MLL/AFX적검측방법。방법운용역전록-다중PCR확증목적조대，탈양핵당핵산측서분석，병대1례급성부분분화형원시립세포백혈병（M2）환자진행료방법학험증。결과해환자MLL/AFX융합기인양성。결론역전록-다중PCR결합측서적방법능구준학지감정한견백혈병융합기인MLL/AFX。해방법시일충령민、쾌속적백혈병융합기인검측방법。
Objective To establish an effective method for MLL/AFX leukemia fusion gene detection.Method After reverse transcription-multiple PCR amplifying,target bands were sequenced.Result The MLL/AFX fusion gene in the patient was positive.Conclusion Reverse transcription-multiple PCR combined with DNA sequencing was a sensitive and rapid method to accurately detect rare fusion gene MLL/AFX.