CAJ | 학술논문

该课题对胚胎期12.5、13.5、14.5d的C57/BL6孕鼠胎鼠以不同的电压参数进行子宫内电穿孔，分析比较不同电压对不同胚胎期胎鼠的存活率，从而总结出适应胚胎期12.5、13.5、14.5d胎鼠的最优电转电压参数。结果显示，胚胎早期，电转所需要的电压相对小一些，同样胚胎早期的胎鼠的存活率低一些。在E14.5胎鼠脑室单侧电穿孔电转带有PCDF-EGFP质粒。该质粒表达绿色荧光蛋白（GFP）。进一步分析了电转后不同天数转染细胞的迁移程度，结果显示，在电转后2d，转染阳性的细胞主要分布在脑室和中间带，而在转染后3d，转染阳性细胞在脑室、中间带及皮层均有分布。继而对电转后的皮层厚度进行了分析，结果显示，电转皮层的厚度与未进行电转的皮层没有明显区别。该结果为子宫内电转在皮层发育中的应用提供了技术支持，并表明子宫内电转可以应用在皮层发育研究中。
해과제대배태기12.5、13.5、14.5d적C57/BL6잉서태서이불동적전압삼수진행자궁내전천공，분석비교불동전압대불동배태기태서적존활솔，종이총결출괄응배태기12.5、13.5、14.5d태서적최우전전전압삼수。결과현시，배태조기，전전소수요적전압상대소일사，동양배태조기적태서적존활솔저일사。재E14.5태서뇌실단측전천공전전대유PCDF-EGFP질립。해질립표체록색형광단백（GFP）。진일보분석료전전후불동천수전염세포적천이정도，결과현시，재전전후2d，전염양성적세포주요분포재뇌실화중간대，이재전염후3d，전염양성세포재뇌실、중간대급피층균유분포。계이대전전후적피층후도진행료분석，결과현시，전전피층적후도여미진행전전적피층몰유명현구별。해결과위자궁내전전재피층발육중적응용제공료기술지지，병표명자궁내전전가이응용재피층발육연구중。
The subjects for embryonic stage 12.5, 13.5, 14.5 the C57/BL6 pregnant mouse fetal to different parameters utero electroporation were analyzed with different voltage of different embryonic period of fetal mouse survival rate,thus to summarize for embryonic day 12.5, 13.5, 14.5,the optimal voltage parameters of fetal mice.The results show that the early embryo,electrical voltage is required relatively small and early embryonic fetal mice survival rate is low.E14.5 fetal mouse ventricular unilateral electroporation transfer with the PCDF-EGFP plasmid,the plasmid expressing green fluorescent protein(GFP).We further analyzed the electrically different days after transfection cells transfected migration degree.The results showed that 2 days after electroporation,transfection-positive cells are mainly distributed in the ventricle and the intermediate zone,3 days after transfection,the transfected positive cells were distributed in the ventricle,the intermediate zone and cortex.We then analyzed cortical thickness.The results show that the electrical cortical thickness is no significant difference.Our results for cortical development in utero electroporation in providing technical support show that in utero electroporation can be applied to the study of cortical development.