化工学报
化工學報
화공학보
JOURNAL OF CHEMICAL INDUSY AND ENGINEERING (CHINA)
2014年
2期
593-598
,共6页
杜巧燕%林东强%王荣柱%姚善泾
杜巧燕%林東彊%王榮柱%姚善涇
두교연%림동강%왕영주%요선경
乳铁蛋白%静态吸附%pH%盐浓度%分子模拟%表面电势%结合能
乳鐵蛋白%靜態吸附%pH%鹽濃度%分子模擬%錶麵電勢%結閤能
유철단백%정태흡부%pH%염농도%분자모의%표면전세%결합능
lactoferrin%adsorption isotherms%pH%salt concentration%molecular simulation%electrostatic potential%binding energy
磺酸基团是用来分离乳铁蛋白普遍被认可的配基,因此含有磺酸基团的层析剂是被用得最广泛的分离介质。通过对三种接有磺酸基团的层析介质,在不同pH和盐浓度时的静态吸附研究,得到了吸附的基本规律:随着pH或盐浓度的增大,吸附容量降低。利用分子模拟技术,比较了乳铁蛋白分子表面电势在不同条件时的变化,推测pH影响吸附的机理在于改变了蛋白表面氨基酸残基的质子化状态和分子表面电势,而盐浓度主要是屏蔽了蛋白和配基之间的相互作用,改变蛋白分子表面整体电势强弱,对电势分布没有明显影响。通过计算蛋白和配基之间的结合能,量化不同条件下的吸附能力,表征pH和盐浓度的影响。
磺痠基糰是用來分離乳鐵蛋白普遍被認可的配基,因此含有磺痠基糰的層析劑是被用得最廣汎的分離介質。通過對三種接有磺痠基糰的層析介質,在不同pH和鹽濃度時的靜態吸附研究,得到瞭吸附的基本規律:隨著pH或鹽濃度的增大,吸附容量降低。利用分子模擬技術,比較瞭乳鐵蛋白分子錶麵電勢在不同條件時的變化,推測pH影響吸附的機理在于改變瞭蛋白錶麵氨基痠殘基的質子化狀態和分子錶麵電勢,而鹽濃度主要是屏蔽瞭蛋白和配基之間的相互作用,改變蛋白分子錶麵整體電勢彊弱,對電勢分佈沒有明顯影響。通過計算蛋白和配基之間的結閤能,量化不同條件下的吸附能力,錶徵pH和鹽濃度的影響。
광산기단시용래분리유철단백보편피인가적배기,인차함유광산기단적층석제시피용득최엄범적분리개질。통과대삼충접유광산기단적층석개질,재불동pH화염농도시적정태흡부연구,득도료흡부적기본규률:수착pH혹염농도적증대,흡부용량강저。이용분자모의기술,비교료유철단백분자표면전세재불동조건시적변화,추측pH영향흡부적궤리재우개변료단백표면안기산잔기적질자화상태화분자표면전세,이염농도주요시병폐료단백화배기지간적상호작용,개변단백분자표면정체전세강약,대전세분포몰유명현영향。통과계산단백화배기지간적결합능,양화불동조건하적흡부능력,표정pH화염농도적영향。
Sulfonate group is the most common ligand used for purifying lactoferrin. The isotherm adsorption curves of lactoferrin on three adsorbents with sulfonic ligand at different pHs and salt concentrations showed that adsorption capacity decreased with the increase of pH or salt concentration. Further molecular simulation was performed to compare the electrostatic potential of lactoferrin under different conditions. It could be presumed that the mechanism of pH effect was the change of protonation of amino acid residues on the surface of protein and the change of electrostatic potential consequently, while the mechanism of salt effect could be the shield of electrostatic interaction between protein and ligand with no impact on electrostatic potential distribution obviously. Finally, the binding energy between protein and ligand under different conditions was calculated to quantify adsorption capacity and characterize the effects of pH and salt concentration.