CAJ | 학술논문

目的：探讨氯化锂对人肝癌细胞BEL-7402增殖、周期的影响及其作用机制。方法应用MTT法及DNA PREP细胞周期法检测BEL-7402经氯化锂作用后的增殖及周期改变情况，Western blot检测糖原合成激酶-3（GSK-3）、失活pGSK-3及其下游分子CyclinD1的表达情况。结果氯化锂可显著抑制BEL-7402的增殖，并呈时间、剂量依赖性（P＜0.05）；氯化锂可使BEL-7402的G0/G1期比例明显降低、G2/M期比例显著升高（P＜0.05）；氯化锂作用后GSK-3、失活pGSK-3及CyclinD1蛋白的表达水平均明显升高（P＜0.05）。结论氯化锂可抑制肝癌细胞增殖，其机制可能是通过抑制GSK-3活性并上调CyclinD1表达使肝癌细胞阻滞于G2/M期。
목적：탐토록화리대인간암세포BEL-7402증식、주기적영향급기작용궤제。방법응용MTT법급DNA PREP세포주기법검측BEL-7402경록화리작용후적증식급주기개변정황，Western blot검측당원합성격매-3（GSK-3）、실활pGSK-3급기하유분자CyclinD1적표체정황。결과록화리가현저억제BEL-7402적증식，병정시간、제량의뢰성（P＜0.05）；록화리가사BEL-7402적G0/G1기비례명현강저、G2/M기비례현저승고（P＜0.05）；록화리작용후GSK-3、실활pGSK-3급CyclinD1단백적표체수평균명현승고（P＜0.05）。결론록화리가억제간암세포증식，기궤제가능시통과억제GSK-3활성병상조CyclinD1표체사간암세포조체우G2/M기。
Objective To investigate the effect of lithium chloride (LiCl) on cellproliferation,cellcycle of human hepatoma cells and its mechanism. Methods Cultured human hepatoma BEL- 7402 cells were treated with different concentrations of LiCl. cellproliferation was measured by MTT assay and cellcycle was examined by DNA PREP cell cycle assay. The expressions of GSK- 3, inactivated GSK- 3 and CyclinD1 were determined by Western blot. Results LiCl inhibited BEL- 7402 cellproliferation in a concentration- and time- dependent manner. After LiCl treatment the percentage of G0/G1 phase was significantly reduced, the G2/M phase was significantly increased, and the expression levels of GSK- 3, inactivated GSK- 3 and CyclinD1 were up- regulat-ed. Conclusion LiCl can inhibit the proliferation of BEL- 7402 cells, in which inactivation of GSK- 3 and up- regulation of CyclinD1 may be involved leading to cellcycle arrest in G2/M phase.