CAJ | 학술논문

先将庆大霉素与载体蛋白偶联形成完全抗原，再与庆大霉素单克隆抗体反应制备试剂条。通过抗原抗体之间的免疫反应，采用胶体金检测方法，将抗原按不同浓度喷制两条检测线，使牛奶中庆大霉素检测灵敏度分别为20μg/kg和100μg/kg，该检测限满足我国及欧盟对庆大霉素检测的要求，并且能够实现牛奶样品中庆大霉素现场快速检测。
선장경대매소여재체단백우련형성완전항원，재여경대매소단극륭항체반응제비시제조。통과항원항체지간적면역반응，채용효체금검측방법，장항원안불동농도분제량조검측선，사우내중경대매소검측령민도분별위20μg/kg화100μg/kg，해검측한만족아국급구맹대경대매소검측적요구，병차능구실현우내양품중경대매소현장쾌속검측。
This study developed a kind of strip which Gentamicin conjugated to a carrier protein to form complete anti-gen,and Gentamicin monoclonal antibody was prepared by the specific reaction.Between the immune reaction,antigens in dif-ferent concentration sprayed two detection lines,make the sensitivity of the test from 20μg/kg to 100μg/kg in milk,and the de-tection limits meet EU and national on Gentamicin testing requirements.The rapid detection of milk sample meets the fast de-tection requirements,and makes the testing of Gentamicin residue from qualitative analysis to semi quantitative detection.