CAJ | 학술논문

假单胞菌（Pseudomonas sp.）YT3和枯草芽孢杆菌（Bacillus subtilis）DZ1是从植物根际土壤筛选出的具有应用潜力的植物根际促生细菌。研究发酵罐中接种量、转速、通气量等因素对 YT3菌量以及对 DZ1芽孢率、菌量的影响。结果表明：不同的接种量、转速和通气量对Pseudomonas sp. YT3的活菌数、Bacillus subtilis DZ1的活菌数及芽孢率均有显著影响。10%的接种量、2 m3/h的通气量对YT3和DZ1菌株的作用效果与15%的接种量、3 m3/h相当，但显著高于5%的接种量和1 m3/h。当转速为200 r/min时，YT3具有最高的活菌数，DZ1具有最高的活菌数和芽孢率。综合分析认为，YT3和DZ1发酵罐的最佳条件为接种量10%、转速200 r/min和通气量2 m3/h。
가단포균（Pseudomonas sp.）YT3화고초아포간균（Bacillus subtilis）DZ1시종식물근제토양사선출적구유응용잠력적식물근제촉생세균。연구발효관중접충량、전속、통기량등인소대 YT3균량이급대 DZ1아포솔、균량적영향。결과표명：불동적접충량、전속화통기량대Pseudomonas sp. YT3적활균수、Bacillus subtilis DZ1적활균수급아포솔균유현저영향。10%적접충량、2 m3/h적통기량대YT3화DZ1균주적작용효과여15%적접충량、3 m3/h상당，단현저고우5%적접충량화1 m3/h。당전속위200 r/min시，YT3구유최고적활균수，DZ1구유최고적활균수화아포솔。종합분석인위，YT3화DZ1발효관적최가조건위접충량10%、전속200 r/min화통기량2 m3/h。
Pseudomonas sp. YT3 and Bacillus subtilis DZ1, isolated and screened from the plant rhizo-sphere soil, were plant growth-promoting rhizo-bacteria exhibiting broad application prospects in the bio-fertilizer production. Taking YT3 and DZ1 bacterial strains as research objects, the experiment were conducted to study the influence of inoculation quantity, rotating speed and minute ventilation on the bacteria population and spore rates of YT3 and DZ1 in the fermentation cylinders. The results indicated that different inoculation quantity, rotating speed and minute ventilation could have significantly effects on the bacteria population and spore rates of YT3 and DZ1. Effects of the 10%inoculation quantity and 2 m3/h ventilation were similar on the bacteria population and spore rates of YT3 and DZ1 with that of the 15% inoculation quantity and 3 m3/h ventilation, however, their effects were higher than that of the 5%inoculation quantity and 2 m3/h ventilation. The numbers of YT3, as well as the numbers and the spore rates of DZ1 reached the highest under 200r/min rotating speed. As a result, the optimum fermentation cylinder conditions of YT3 and DZ1 were 10% inoculation quantity, 200 r/min rotating speed and 2 m3/h ventilation.