CAJ | 학술논문

目的：观察标本采集、送检、放置时间、温度对凝血四项测定结果的影响，为建立检测流程提供依据。方法对外科住院患者65例，病房早6：00前采集标本30min内送检，检验科即刻分离血浆标本检测凝血酶原时间（PT），纤维蛋白原（FIB），活化部分凝血活酶时间（APTT）和凝血酶时间（TT）；其余标本分别保存4℃与室温在采血后2h，3．5h，4h，8h，16h检测，与即刻测定结果进行配对分析。结果即刻测定结果与4℃、室温（20～25℃）放置2h和3．5h，4h，8h，16h测定结果比较，TT和APTT测定结果差异有统计学意义（P＜0．01）。结论凝血四项标本室温、4℃放置过久与即刻测定结果相比，差异有显著性（P＜0．01）。 TT测定结果缩短和APTT测定结果延长；标本放置室温、4℃分别在采血后2h，4h内完成。
목적：관찰표본채집、송검、방치시간、온도대응혈사항측정결과적영향，위건립검측류정제공의거。방법대외과주원환자65례，병방조6：00전채집표본30min내송검，검험과즉각분리혈장표본검측응혈매원시간（PT），섬유단백원（FIB），활화부분응혈활매시간（APTT）화응혈매시간（TT）；기여표본분별보존4℃여실온재채혈후2h，3．5h，4h，8h，16h검측，여즉각측정결과진행배대분석。결과즉각측정결과여4℃、실온（20～25℃）방치2h화3．5h，4h，8h，16h측정결과비교，TT화APTT측정결과차이유통계학의의（P＜0．01）。결론응혈사항표본실온、4℃방치과구여즉각측정결과상비，차이유현저성（P＜0．01）。 TT측정결과축단화APTT측정결과연장；표본방치실온、4℃분별재채혈후2h，4h내완성。
Objective To observe the effect of specimen collection ,submission,storage time and temperature on coagulation de-termination results ,and to provide the basis for the establishment of testing processes .Methods Specimen from 65 Surgical ward patients was collected before 6:00 o'clock,and sent in 30 minutes for the inspection of prothrombin time (PT),fibrinogen(FIB),activated partial thromboplastintime(APTT) and thrombin time(TT) immediately.The remaining samples were stored in 4℃ and in room temperature and were testedafter 2h,3.5h,4h,8h,16h respectively.The matched -pair analysis was took immediately.Results According to the results,the differencesof TT and APTT showed statistical meaning (P <0.01).Conclusion The specimens placed at room temperature for too long ,themeasurement results of TT will be shortened ,while the APTT measurement results will be extended.The differences between Immediate determinationresults and results of measuring specimens at room temperature and 4℃ are obvious.