CAJ | 학술논문

本文建立了橄榄油中角鲨烯的提取及检测方法。研究形成以橄榄油为原料，利用酯化分离得到橄榄油中的不可皂化物经柱层析吸附分离纯化得到角鲨烯，并对该角鲨烯进行HPLC 分析。结果显示：该橄榄油中角鲨烯的含量为569.21mg/100g，且提取所得角鲨烯纯度为84.33%，对高纯度角鲨烯的生产有着重要指导作用；该HPLC检测方法在0.2-1.8mg/mL范围内线性关系良好，相关系数为0.9970，相对标准偏差为1.04%，方法回收率较高，定量准确，灵敏度高，可用于角鲨烯的定量分析。
본문건립료감람유중각사희적제취급검측방법。연구형성이감람유위원료，이용지화분리득도감람유중적불가조화물경주층석흡부분리순화득도각사희，병대해각사희진행HPLC 분석。결과현시：해감람유중각사희적함량위569.21mg/100g，차제취소득각사희순도위84.33%，대고순도각사희적생산유착중요지도작용；해HPLC검측방법재0.2-1.8mg/mL범위내선성관계량호，상관계수위0.9970，상대표준편차위1.04%，방법회수솔교고，정량준학，령민도고，가용우각사희적정량분석。
In this paper, an efficient extraction and detection method of squalene derived from olive oil was proposed. After the esterification reaction from the olive oil, the obtained unsaponifiable substance was further purified by the chromatography column to get saqualane, which content was determined by HPLC. Results showed that the squalene content of this olive oil reached 569.21mg/100g, and the purity was 84.33%. HPLC detection method showed a good linear relationship in the range of 0.2~1.8mg/mL, and the correlation coefficient was 0. 9970, and relative standard deviation reached 1.04%. Additionally, it can be suggested that the method exhibited high accuracy, sensitivity and recovery, which could be applied to quantitative analysis of squalene.