华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
5期
138-143
,共6页
张志刚%李巧云%刘栓桃%张晓亮%赵智中
張誌剛%李巧雲%劉栓桃%張曉亮%趙智中
장지강%리교운%류전도%장효량%조지중
大白菜%芜菁花叶病毒%酶活性%H2O2
大白菜%蕪菁花葉病毒%酶活性%H2O2
대백채%무정화협병독%매활성%H2O2
Chinese cabbage%TuMV%Enzyme activity%H2O2
选取抗TuMV的8407、河304和感TuMV的冠291和春月黄为试验材料,于苗期接种TuMV-C4,接种后测定24 d内叶片中超氧化物岐化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)这3种过氧化氢代谢相关酶的活性以及过氧化氢H2 O2的含量。结果表明:接种TuMV后, POD、CAT的活性及H2 O2含量的变化在不同材料之间存在明显差异。抗病材料在接种后,POD、CAT的活性及H2 O2含量虽有变化,但均能逐渐恢复正常;感病材料在接种后,POD、CAT的活性及H2 O2含量均有较大变化,且始终无法恢复正常。总体而言,叶片中的H2 O2和CAT与大白菜的TuMV抗性关系较为紧密,其次是POD,而SOD与TuMV抗性的关系不大。
選取抗TuMV的8407、河304和感TuMV的冠291和春月黃為試驗材料,于苗期接種TuMV-C4,接種後測定24 d內葉片中超氧化物岐化酶(SOD)、過氧化物酶(POD)、過氧化氫酶(CAT)這3種過氧化氫代謝相關酶的活性以及過氧化氫H2 O2的含量。結果錶明:接種TuMV後, POD、CAT的活性及H2 O2含量的變化在不同材料之間存在明顯差異。抗病材料在接種後,POD、CAT的活性及H2 O2含量雖有變化,但均能逐漸恢複正常;感病材料在接種後,POD、CAT的活性及H2 O2含量均有較大變化,且始終無法恢複正常。總體而言,葉片中的H2 O2和CAT與大白菜的TuMV抗性關繫較為緊密,其次是POD,而SOD與TuMV抗性的關繫不大。
선취항TuMV적8407、하304화감TuMV적관291화춘월황위시험재료,우묘기접충TuMV-C4,접충후측정24 d내협편중초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)저3충과양화경대사상관매적활성이급과양화경H2 O2적함량。결과표명:접충TuMV후, POD、CAT적활성급H2 O2함량적변화재불동재료지간존재명현차이。항병재료재접충후,POD、CAT적활성급H2 O2함량수유변화,단균능축점회복정상;감병재료재접충후,POD、CAT적활성급H2 O2함량균유교대변화,차시종무법회복정상。총체이언,협편중적H2 O2화CAT여대백채적TuMV항성관계교위긴밀,기차시POD,이SOD여TuMV항성적관계불대。
The 4 materials 8407(R),He304(R),Guan291(S) and Chunyuehuang(S) which have different re-sistance to TuMV ,were chosen to be the material plants .They were inoculated with TuMV-C4 at seedling stage .Activi-ties of SOD,POD,CAT and H2 O2 content from 4 materials were detected within 24 days after being inoculated with TuMV.The results showed that significant differences in the changes of enzymes activities of POD ,CAT and H2 O2 content existed in different materials .The enzymes activities of POD,CAT and H2 O2 content in resistant materials had some changes with TuMV infection ,but could gradually return to normal .The enzymes activities of POD ,CAT and H2 O2 content in susceptible materials all had significant changes ,and could not return to normal after being in-oculate with TuMV .Overall TuMV resistance of Chinese cabbage had the most closely relationship with H 2 O2 and CAT,and had less relationship with POD ,and had no relationship with SOD .