CAJ | 학술논문

为验证花粉管通道法进行蓖麻转基因研究的可行性，优化花粉管通道法导入方式及导入时间，为花粉管通道法转基因技术在蓖麻分子育种的应用提供参考依据。采用荧光显微观察对蓖麻品种通蓖5号人工授粉后花粉萌发及花粉管生长情况进行了研究，结果表明，花粉管通道法转化蓖麻的最佳时间为授粉后6～24 h。利用花粉管通道技术，将含有GUS基因的植物双元表达载体pPZP221 SGN导入蓖麻品种通蓖5号。对85株T0植株进行PCR检测，得到10株阳性转化植株，阳性率约为11．8％。
위험증화분관통도법진행비마전기인연구적가행성，우화화분관통도법도입방식급도입시간，위화분관통도법전기인기술재비마분자육충적응용제공삼고의거。채용형광현미관찰대비마품충통비5호인공수분후화분맹발급화분관생장정황진행료연구，결과표명，화분관통도법전화비마적최가시간위수분후6～24 h。이용화분관통도기술，장함유GUS기인적식물쌍원표체재체pPZP221 SGN도입비마품충통비5호。대85주T0식주진행PCR검측，득도10주양성전화식주，양성솔약위11．8％。
The study on the pollen germination and pollen tube growth of castor was performed after artificial pollination via fluorescence microscopy observation in order to verify that the best time to perform genetic transfor -mation of castor by pollen-tube pathway method is 6-24 h after pollination .Plant expression vector pPZP 221 SGN contained gene GUS were transformed into castor varieties tongbi 5 through pollen-tube pathway .85 strains of T0 gen-eration were tested by PCR method and the positive strains is 10,positive rate is 11.8%.The results show it is pos-sible to perform transgenic by pollen-tube pathway .This provides some references for the application of transforma-tion technology by pollen-tube pathway in castor molecular breeding .