CAJ | 학술논문

水稻稻瘟病是最具毁灭性的病害之一，严重地影响水稻的高产和稳产。在病原菌侵染水稻时，附着胞的形成对稻瘟病菌的致病性起着关键作用。研究证实一种P型ATP酶（ P-ATPase）参与了附着胞的形成。在病原菌与寄主的互作过程中，寄主的一些小分子物质可以进入病原菌中，达到抗病原菌侵染的目的。以稻瘟菌致病关键的P-AT-Pase基因MgAPT2第一外显子上特异性好的232 bp的区域作为干扰片段，正反向插入干扰载体中，通过农杆菌介导，转化到感稻瘟病水稻品种日本晴中，通过苗期稻瘟病接种鉴定和MgAPT2基因的表达检测，结果表明：转基因植株稻瘟病抗性得到增强且稻瘟病菌MgAPT2基因的表达量下降，为水稻抗稻瘟病种质资源的创新提供了新思路。
수도도온병시최구훼멸성적병해지일，엄중지영향수도적고산화은산。재병원균침염수도시，부착포적형성대도온병균적치병성기착관건작용。연구증실일충P형ATP매（ P-ATPase）삼여료부착포적형성。재병원균여기주적호작과정중，기주적일사소분자물질가이진입병원균중，체도항병원균침염적목적。이도온균치병관건적P-AT-Pase기인MgAPT2제일외현자상특이성호적232 bp적구역작위간우편단，정반향삽입간우재체중，통과농간균개도，전화도감도온병수도품충일본청중，통과묘기도온병접충감정화MgAPT2기인적표체검측，결과표명：전기인식주도온병항성득도증강차도온병균MgAPT2기인적표체량하강，위수도항도온병충질자원적창신제공료신사로。
Rice blast disease is the severest disease in rice production .The formation of appressorium plays a key role on disease extending when pathogenic fungi intrudes into rice .The formation of appressorium needs a P-type ATPase .Small molecules from the host are able to enter into the pathogen during the interactions between pathogen and host ,to reach the purpose of anti-pathogen infection .In this study ,a 232 bp DNA sequence from the first exon of the P-ATPase gene,MgAPT2,was used as interfering fragment and inserted into interfering vector posi-tively and negatively .The interfering vector was then transformed into rice variety Nipponbare susceptible to rice blast.Rice blast resistance of transgenic plants and gene expression were checked .The results showed that the ex-pression level of the P-ATPase gene MgAPT2 from Magnaporthe grisea in transgenic rice decreased and blast-dis-ease resistance of transgenic rice increased .This study provided a new way for innovation of rice blast-disease resist-ance germplasm .