华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
5期
101-105
,共5页
李芦江%熊尧宇%陈文生%兰海%林海建%杨克诚%高世斌%潘光堂
李蘆江%熊堯宇%陳文生%蘭海%林海建%楊剋誠%高世斌%潘光堂
리호강%웅요우%진문생%란해%림해건%양극성%고세빈%반광당
玉米%自交系%回交%选择方向%08-641%遗传变异%SSR
玉米%自交繫%迴交%選擇方嚮%08-641%遺傳變異%SSR
옥미%자교계%회교%선택방향%08-641%유전변이%SSR
Maize%Inbred lines%Backcross breeding%Select direction%08-641%Genetic variation%SSR
利用SSR分子标记对玉米自交系08-641( R08)不同选择方向回交改良得到的79个BC2 F4后代的遗传多样性进行分析。结果表明,利用44对具有清晰多态性的引物共检测到272个多态性等位基因位点,其中有123个位点在回交后代株系中被检测到而在轮回亲本中缺失;以种子形状和颜色不像R08为标准选择得到的回交后代株系,基因型与R08的相似程度总体低于以种子形状和颜色类似R08为标准选择得到的回交后代株,且其变异范围更广。可见不同选择方向所得回交后代选系的遗传变异存在一定的差异。因此,在回交过程中采用多向选择的策略,可能有利于提高回交改良的育种效率。
利用SSR分子標記對玉米自交繫08-641( R08)不同選擇方嚮迴交改良得到的79箇BC2 F4後代的遺傳多樣性進行分析。結果錶明,利用44對具有清晰多態性的引物共檢測到272箇多態性等位基因位點,其中有123箇位點在迴交後代株繫中被檢測到而在輪迴親本中缺失;以種子形狀和顏色不像R08為標準選擇得到的迴交後代株繫,基因型與R08的相似程度總體低于以種子形狀和顏色類似R08為標準選擇得到的迴交後代株,且其變異範圍更廣。可見不同選擇方嚮所得迴交後代選繫的遺傳變異存在一定的差異。因此,在迴交過程中採用多嚮選擇的策略,可能有利于提高迴交改良的育種效率。
이용SSR분자표기대옥미자교계08-641( R08)불동선택방향회교개량득도적79개BC2 F4후대적유전다양성진행분석。결과표명,이용44대구유청석다태성적인물공검측도272개다태성등위기인위점,기중유123개위점재회교후대주계중피검측도이재륜회친본중결실;이충자형상화안색불상R08위표준선택득도적회교후대주계,기인형여R08적상사정도총체저우이충자형상화안색유사R08위표준선택득도적회교후대주,차기변이범위경엄。가견불동선택방향소득회교후대선계적유전변이존재일정적차이。인차,재회교과정중채용다향선택적책략,가능유리우제고회교개량적육충효솔。
In this study ,SSR molecular markers were used to analysis the genetic variation of 79 backcross modi-fied lines of the maize inbred line 08-641(R08).The result showed that a total of 272 alleles were detected in the im-proved lines and R08,and 123 of them were detected in the modified lines but discarded in R 08.The modified line se-lected based on simialry in the shape and corlor of seeds to R 08 have lower genetic similary between R 08 than that between the lines selected based on dissimialry in the shape and corlor of seeds and R 08,and the genetic varioation of theses lines were wider .It conduvted that when the backcross breeding were used to improve the maize inbred lines , multidirectional selection based on phenotypic value were contribute to create and keep genetic variation .