CAJ | 학술논문

从棉花转BT-CPTI双价抗虫基因后代中选育了核不育种质98-8A，采用表型观察、自交和测交手段，对其植株表型、花器、育性特征及其遗传规律进行了系统分析。花器官形态观察表明，不育株花柱长和花柱外露长度均明显高于同质系的正常可育株，花丝明显萎缩，不育株花药无花粉粒出现，而子房直径及花药数量没有明显差异；对不育株与可育株姊妹交后代及可育株自交后代遗传分析表明，该雄性不育性受隐性单基因控制；等位性检测显示，98-8 A的不育基因与已知的473A（ms14/msc1）、1355A（ms2/msc2）、阆A（ms15/msc3）等3个不育基因为非等位基因。不育株与常规多品种杂交分析表明，98-8 A的一般配合力与特殊配合力均较高，且衣分高、纤维品质较好，暗示有较好的应用前景。
종면화전BT-CPTI쌍개항충기인후대중선육료핵불육충질98-8A，채용표형관찰、자교화측교수단，대기식주표형、화기、육성특정급기유전규률진행료계통분석。화기관형태관찰표명，불육주화주장화화주외로장도균명현고우동질계적정상가육주，화사명현위축，불육주화약무화분립출현，이자방직경급화약수량몰유명현차이；대불육주여가육주자매교후대급가육주자교후대유전분석표명，해웅성불육성수은성단기인공제；등위성검측현시，98-8 A적불육기인여이지적473A（ms14/msc1）、1355A（ms2/msc2）、랑A（ms15/msc3）등3개불육기인위비등위기인。불육주여상규다품충잡교분석표명，98-8 A적일반배합력여특수배합력균교고，차의분고、섬유품질교호，암시유교호적응용전경。
Phenotype and microscopic observation ,classical self hybridyzation and sib-cross means were applied to analyze the flower phenotypic characteristics ,genetic model,and the allelism for Yu 98-8A line cotton genic male sterile line bred from the offspring population of a genetically modified cotton population .Floral morphology investi-gation showed that ,the length of style and exposed style of sterile plants were significantly higher than that of homoge -neity fertile plants ,filaments shriveled markedly ,and there was no pollen within the anther of sterile plants ,while the diameter of ovary and the number of anthers of each flower had no significant difference .Genetic analysis of hybrid offspring population of sterile and heterozygous fertile plants and the self -cross of fertile heterozygous plants showed that the male sterility was controlled by a single recessive gene .Allelic detection analysis implied that the male sterile gene of Yu 98-8A was different from ms14,ms2 and ms15.Analysis of hybrid offspring population of Yu 98-8A and conventional cultivars showed that not only both of the general and specific combining abilities were higher ,but also the lint percentage and fiber quality were better ,all of which suggested a better application prospect .