CAJ | 학술논문

为了提高毛霉产中性蛋白酶酶活力，通过单因素实验和正交实验对毛霉发酵培养条件进行优化，结果表明，雅致放射毛霉产中性蛋白酶最佳培养条件为：培养时间72 h、培养温度30℃、pH6.8、接种量9%，此条件下中性蛋白酶酶活力达1967.36 U/mL；五通桥毛霉产中性蛋白酶最佳培养条件为：培养温度27℃、培养时间78 h、pH6.5、接种量7%，此条件下中性蛋白酶酶活力达1896.53 U/mL。
위료제고모매산중성단백매매활력，통과단인소실험화정교실험대모매발효배양조건진행우화，결과표명，아치방사모매산중성단백매최가배양조건위：배양시간72 h、배양온도30℃、pH6.8、접충량9%，차조건하중성단백매매활력체1967.36 U/mL；오통교모매산중성단백매최가배양조건위：배양온도27℃、배양시간78 h、pH6.5、접충량7%，차조건하중성단백매매활력체1896.53 U/mL。
In order to improve the neutral protease activity of mucor, optimization of culture condition during mucor fermentation was examined. Based on the single-factor experiment and orthogonal experiment, the optimized culture conditions were obtained. For the neutral protease production of Actinomucor elegans, the incubation time was 72 h, incubation temperature was 30 ℃, pH6.8 and the amount of mucor inoculated spores was 9%, the neutral protease activity was 1 967. 36 U/mL; For the neutral protease production of Wutungkiao Mucor, the culture temperature was 27℃, incubation time was 78 h, pH6.5 and the amount of mucor inoculated spores was 7%with protease activity of 1 896.53 U/mL.