医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2013年
7期
257-258
,共2页
张波%廖刚%孔宪炳%张国武
張波%廖剛%孔憲炳%張國武
장파%료강%공헌병%장국무
CO2气腹%兔肝VX2肿瘤%血管新生%基质金属蛋白酶
CO2氣腹%兔肝VX2腫瘤%血管新生%基質金屬蛋白酶
CO2기복%토간VX2종류%혈관신생%기질금속단백매
CO2 pneumoperitoneum%Rabbit liver VX2 tumor%Angiogenesis%MMP
目的探讨CO2气腹对兔肝VX2肿瘤MMP2及血管新生的影响。方法采用组织块移植法建立兔肝 VX2肿瘤模型30只，随机分成3组，即气腹组(A组，n=10)、基质金属蛋白酶抑制剂(MMPi)组(B组，n=10)及免气腹组(C组，n=10)，模拟腹腔镜手术环境。免疫组织化学方法检测肿瘤及肿瘤旁组织MMP2及CD34的表达，采用IPP图像分析软件分析其平均光密度。结果 MMP2表达：A组、B组、C组肿瘤组织 MMP2平均光密度分别为0.2052±0.0167，0.1980±0.0332，0.1711±0.0284(P=0.022)；肿瘤旁组织 MMP2平均光密度分别为0.2081±0.0199，0.1726±0.0073，0.1396±0.0079(P=0.000)。CD34表达：A组、B组、C组肿瘤组织CD34平均光密度分别为0.2310±0.0226，0.2219±0.0154，0.1886±0.0180(P=0.000)；肿瘤旁组织CD34平均光密度分别为0.2198±0.0505，0.1761±0.0237，0.1108±0.0046(P=0.000)。结论 CO2气腹可通过 MMP2促进兔肝VX2肿瘤血管新生。
目的探討CO2氣腹對兔肝VX2腫瘤MMP2及血管新生的影響。方法採用組織塊移植法建立兔肝 VX2腫瘤模型30隻，隨機分成3組，即氣腹組(A組，n=10)、基質金屬蛋白酶抑製劑(MMPi)組(B組，n=10)及免氣腹組(C組，n=10)，模擬腹腔鏡手術環境。免疫組織化學方法檢測腫瘤及腫瘤徬組織MMP2及CD34的錶達，採用IPP圖像分析軟件分析其平均光密度。結果 MMP2錶達：A組、B組、C組腫瘤組織 MMP2平均光密度分彆為0.2052±0.0167，0.1980±0.0332，0.1711±0.0284(P=0.022)；腫瘤徬組織 MMP2平均光密度分彆為0.2081±0.0199，0.1726±0.0073，0.1396±0.0079(P=0.000)。CD34錶達：A組、B組、C組腫瘤組織CD34平均光密度分彆為0.2310±0.0226，0.2219±0.0154，0.1886±0.0180(P=0.000)；腫瘤徬組織CD34平均光密度分彆為0.2198±0.0505，0.1761±0.0237，0.1108±0.0046(P=0.000)。結論 CO2氣腹可通過 MMP2促進兔肝VX2腫瘤血管新生。
목적탐토CO2기복대토간VX2종류MMP2급혈관신생적영향。방법채용조직괴이식법건립토간 VX2종류모형30지，수궤분성3조，즉기복조(A조，n=10)、기질금속단백매억제제(MMPi)조(B조，n=10)급면기복조(C조，n=10)，모의복강경수술배경。면역조직화학방법검측종류급종류방조직MMP2급CD34적표체，채용IPP도상분석연건분석기평균광밀도。결과 MMP2표체：A조、B조、C조종류조직 MMP2평균광밀도분별위0.2052±0.0167，0.1980±0.0332，0.1711±0.0284(P=0.022)；종류방조직 MMP2평균광밀도분별위0.2081±0.0199，0.1726±0.0073，0.1396±0.0079(P=0.000)。CD34표체：A조、B조、C조종류조직CD34평균광밀도분별위0.2310±0.0226，0.2219±0.0154，0.1886±0.0180(P=0.000)；종류방조직CD34평균광밀도분별위0.2198±0.0505，0.1761±0.0237，0.1108±0.0046(P=0.000)。결론 CO2기복가통과 MMP2촉진토간VX2종류혈관신생。
Objective To explore the effect of CO2 pneumoperitoneum on expression of MMP2 and angiogenesis of VX2 tumor in rabbit liver.Methods Thirty male New Zealand white rabbits with implanted liver VX2 tumors were randomly divided into three groups,Group A (Pneumo- peritoneum group, n=10), Group B (MMPi group, n=10) and Group C (Pneumo- peritoneum-free group, n=10). All groups received sham laparoscopic operation. Detected MMP2 and CD34 expressions both in tumor tissue and paratumor tissue by immunohisto- chemistry stain. Their mean optical density were analyzed by IPP system (Image-pro-plus 5.0.2). Results Expressions of MMP2 protein:In Group A, B, C, respectively, the mean optical density was 0.2052 ±0.0167,0.1980 ±0.0332,0.1711 ±0.0284 (P=0.022) in tumor tissue and 0.2081 ±0.0199, 0.1726 ±0.0073, 0.1396 ±0.0079 (P=0.000) in paratumor tissue. Expressions of CD34 protein:In Group A, B, C, respectively, the mean optical density was 0.2310±0.0226, 0.2219±0.0154, 0.1886± 0.0180 (P=0.000) in tumor tissue and 0.2198 ±0.0505, 0.1761 ±0.0237, 0.1108 ±0.0046 (P=0.000) in paratumor tissue. Conclusion CO2 pneumoperitoneum stimulates angiogenesis of VX2 tumor in rabbit liver through the change of expressions of MMP2.
