CAJ | 학술논문

目的：观察不同浓度胆固醇对滋养细胞凋亡及尿酸产生的影响。方法：不同胆固醇浓度培养基培养滋养细胞（HPT-8系）24 h后，用Annexin V/PI双染色法检测不同胆固醇浓度培养基中滋养细胞的凋亡程度。同时用酶联免疫吸附（ELISA）法检测不同胆固醇浓度培养滋养细胞24 h后上清液中尿酸的浓度。结果：通过检测分析发现，培养基中胆固醇浓度（0，25，50，100 mg/L）越高，滋养细胞凋亡率越高，细胞培养上清液中的尿酸浓度也越高，且呈现出剂量依赖性。结论：培养基中胆固醇浓度越高，引起滋养细胞凋亡的能力越强，从而导致滋养细胞微粒的数量增加。同时高胆固醇浓度也会引起滋养细胞产生尿酸的水平增加，引起系统炎症反应及血管内皮系统功能的紊乱，从而在子痫前期的发病过程中发挥作用。
목적：관찰불동농도담고순대자양세포조망급뇨산산생적영향。방법：불동담고순농도배양기배양자양세포（HPT-8계）24 h후，용Annexin V/PI쌍염색법검측불동담고순농도배양기중자양세포적조망정도。동시용매련면역흡부（ELISA）법검측불동담고순농도배양자양세포24 h후상청액중뇨산적농도。결과：통과검측분석발현，배양기중담고순농도（0，25，50，100 mg/L）월고，자양세포조망솔월고，세포배양상청액중적뇨산농도야월고，차정현출제량의뢰성。결론：배양기중담고순농도월고，인기자양세포조망적능력월강，종이도치자양세포미립적수량증가。동시고담고순농도야회인기자양세포산생뇨산적수평증가，인기계통염증반응급혈관내피계통공능적문란，종이재자간전기적발병과정중발휘작용。
Objective:To study the influence of different concentrations of cholesterol on cell apoptosis and producing uric acid of trophoblast cell. Methods:Primary culture trophoblasts cells (HPT-8) in the medium with different concentrations of cholesterol for 24 hours,then Annexin V/PI method were used to detect different cells′apoptosis. Meanwhile,we used ELISA to detect uric acid concentration in the supernatant after 24 hours. Results:Through the analysis we found that the higher cholesterol concentrations (0,25,50,100 mg/L) in medium,the higher cell apoptosis rate. The level of uric acid in the cell culture supernatant is higher,and presents a dose dependent. Conclusions:When medium cholesterol concentration become high,it cause more serious apoptosis of cells,which leads to increased number of microparticles. At the same time the high cholesterol concentration can also cause increased production of uric acid ,then induce system inflammatory response and endothelal dysfunction. The two factors play important roles in the pathogenesis of preeclampsia.