CAJ | 학술논문

目的观察微小RNA(miR)-155在乳腺癌中的表达及对乳腺癌HBL-100细胞株迁移能力的影响.方法通过实时定量逆转录聚合酶链反应(RT-qPCR)法检测60例正常乳腺组织及60例乳腺癌组织中miR-155的表达水平,分析其与临床病理特征的关系.同时在乳腺癌HBL-100细胞株中分别转染miR-155-mimics及抑制物,采用Transwell小室实验检测细胞的迁移能力.结果 miR-155在乳腺癌中的表达明显高于正常乳腺组织(P＜0.01),其表达与淋巴结转移及TNM分期明显相关(P＜0.05).Transwell实验检测结果表明miR-155过表达后,细胞的迁移数为(46.3±4.6)个,明显高于对照组[(31.6±2.2)个,P＜0.05];而miR-155表达下调后,细胞的迁移数明显减少,为(20.0±1.9)个(P＜0.05).结论 miR-155表达与乳腺癌的形成密切相关,并影响细胞的迁移能力.
목적 관찰미소RNA(miR)-155재유선암중적표체급대유선암HBL-100세포주천이능력적영향.방법 통과실시정량역전록취합매련반응(RT-qPCR)법검측60례정상유선조직급60례유선암조직중miR-155적표체수평,분석기여림상병리특정적관계.동시재유선암HBL-100세포주중분별전염miR-155-mimics급억제물,채용Transwell소실실험검측세포적천이능력.결과 miR-155재유선암중적표체명현고우정상유선조직(P＜0.01),기표체여림파결전이급TNM분기명현상관(P＜0.05).Transwell실험검측결과표명miR-155과표체후,세포적천이수위(46.3±4.6)개,명현고우대조조[(31.6±2.2)개,P＜0.05];이miR-155표체하조후,세포적천이수명현감소,위(20.0±1.9)개(P＜0.05).결론 miR-155표체여유선암적형성밀절상관,병영향세포적천이능력.
Objective To investigate the expression of microRNA (miR)-155 in breast cancer and its effect on the migration in HBL-100 cell line.Methods Real-time quantitative reverse polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-155 mRNA in 60 cases of breast cancer tissues and 60 cases of normal breast tissues.The relationship between the miR-155 expression and clinicopathological characteristics was analyzed in breast cancer.Tfter transfection of miR-155 mimics and inhibitor into HBL-100 cells respectively,the ability of migration was evaluated by Transwell assay.Results The miR-155 expression in breast cancer tissues was significantly higher than that in normal breast tissues (P ＜0.01).The results of Transwell assay showed that the number of migrating cells in high miR-155 expression group was 46.3 ±4.6,significantly greater than that in control group (31.6 ±2.2) (P ＜0.05).After miR-155 down-regulation,the number of migrating cells was strongly reduced (20.0 ± 1.9) (P ＜0.05).Conclusion miR-155 expression is closely related to the formation of breast cancer,and also affects cell migration.