CAJ | 학술논문

目的建立人乳房脂肪来源干细胞(hbASCs)分离、培养的方法,并研究其形态特点、生物学特性及表面相关标志.方法取乳房缩小术中切除乳腺组织内脂肪,采用Ⅰ型胶原酶消化贴壁法分离原代脂肪来源干细胞,倒置相差显微镜观察细胞形态特点、生物学特性,透射电子显微镜观察细胞超微结构,采用细胞计数试剂盒(CCK-8)法绘制细胞生长曲线,以免疫荧光法和流式细胞仪对其表面标志物进行鉴定,并向成脂、成骨定向诱导.结果原代hbASCs呈长梭形贴壁生长,呈漩涡样,经传代后形态均一.经冻存复苏后存活率达85.7％,仍可维持较好的细胞活性、生长和增殖能力.可阳性表达间充质干细胞生长特性相对特异性标志物CD44、CD105,经油红O和茜素红染色鉴定证实体外可成脂、成骨定向分化.结论可从人乳腺脂肪组织中成功分离获取脂肪组织来源干细胞,为组织工程化乳房提供了取材方便、不增加供区的种子细胞来源.
목적 건립인유방지방래원간세포(hbASCs)분리、배양적방법,병연구기형태특점、생물학특성급표면상관표지.방법 취유방축소술중절제유선조직내지방,채용Ⅰ형효원매소화첩벽법분리원대지방래원간세포,도치상차현미경관찰세포형태특점、생물학특성,투사전자현미경관찰세포초미결구,채용세포계수시제합(CCK-8)법회제세포생장곡선,이면역형광법화류식세포의대기표면표지물진행감정,병향성지、성골정향유도.결과 원대hbASCs정장사형첩벽생장,정선와양,경전대후형태균일.경동존복소후존활솔체85.7％,잉가유지교호적세포활성、생장화증식능력.가양성표체간충질간세포생장특성상대특이성표지물CD44、CD105,경유홍O화천소홍염색감정증실체외가성지、성골정향분화.결론 가종인유선지방조직중성공분리획취지방조직래원간세포,위조직공정화유방제공료취재방편、불증가공구적충자세포래원.
Objective To establish a method for isolating human breast adipose-derived stem cells (hbASCs).Methods hbASCs were isolated,cultured,and expanded from human hypertrophy breast adipose tissue.Immunofluorescent staining,fluorescence-activated cell sorter and multilineage differentiation induction were performed to characterize the obtained cells.Results hbASCs obtained in this study had characteristics of mesenchymal stem cells and expressed specific surface markers.They also possessed a multilineage differentiation potential.Conclusion hbASCs can be isolated from human hypertrophy breast adipose tissue,and they may represent aternative seed cells for tissue engineering.