中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2564-2566
,共3页
胃腺癌%基质金属蛋白酶-6%增殖
胃腺癌%基質金屬蛋白酶-6%增殖
위선암%기질금속단백매-6%증식
Gastric%Adenocarcinoma%Matrix metalloproteinase-6%Proliferation
目的 观察胃腺癌组织中基质金属蛋白酶-6(MMP-6)的表达及沉默MMP-6小干扰RNA(siRNA)对胃腺癌SGC7901细胞增殖的干扰.方法 采用酶联免疫吸附试验检测90例胃腺癌患者的癌组织以及癌旁胃黏膜组织中的MMP-6蛋白的表达,研究其与临床病理特征之间的关系;SGC7901细胞转染MMP-6 siRNA后,采用流式细胞术和噻唑蓝(MTT)法检测转染后细胞的增殖状况及细胞周期.结果 MMP-6蛋白在胃腺癌组织中的阳性表达率(67.9％,62/90)高于癌旁组织(27.1％,24/90;x2=14.812,P＜0.05).胃腺癌组织中MMP-6的表达与淋巴结转移及TNM分期密切相关(P＜0.05)).MMP-6 siRNA转染SGC7901细胞后,细胞增殖活性降低;G1期细胞增多,S期细胞减少(F=14.459、11.813,P＜0.05).结论 胃腺癌的发生、发展可能与MMP-6有关.
目的 觀察胃腺癌組織中基質金屬蛋白酶-6(MMP-6)的錶達及沉默MMP-6小榦擾RNA(siRNA)對胃腺癌SGC7901細胞增殖的榦擾.方法 採用酶聯免疫吸附試驗檢測90例胃腺癌患者的癌組織以及癌徬胃黏膜組織中的MMP-6蛋白的錶達,研究其與臨床病理特徵之間的關繫;SGC7901細胞轉染MMP-6 siRNA後,採用流式細胞術和噻唑藍(MTT)法檢測轉染後細胞的增殖狀況及細胞週期.結果 MMP-6蛋白在胃腺癌組織中的暘性錶達率(67.9％,62/90)高于癌徬組織(27.1％,24/90;x2=14.812,P＜0.05).胃腺癌組織中MMP-6的錶達與淋巴結轉移及TNM分期密切相關(P＜0.05)).MMP-6 siRNA轉染SGC7901細胞後,細胞增殖活性降低;G1期細胞增多,S期細胞減少(F=14.459、11.813,P＜0.05).結論 胃腺癌的髮生、髮展可能與MMP-6有關.
목적 관찰위선암조직중기질금속단백매-6(MMP-6)적표체급침묵MMP-6소간우RNA(siRNA)대위선암SGC7901세포증식적간우.방법 채용매련면역흡부시험검측90례위선암환자적암조직이급암방위점막조직중적MMP-6단백적표체,연구기여림상병리특정지간적관계;SGC7901세포전염MMP-6 siRNA후,채용류식세포술화새서람(MTT)법검측전염후세포적증식상황급세포주기.결과 MMP-6단백재위선암조직중적양성표체솔(67.9％,62/90)고우암방조직(27.1％,24/90;x2=14.812,P＜0.05).위선암조직중MMP-6적표체여림파결전이급TNM분기밀절상관(P＜0.05)).MMP-6 siRNA전염SGC7901세포후,세포증식활성강저;G1기세포증다,S기세포감소(F=14.459、11.813,P＜0.05).결론 위선암적발생、발전가능여MMP-6유관.
Objective To analyze the expression of matrix metalloproteinase (MMP)-6 protein in gastric adenocarcinoma tissue and effect of silent MMP-6 small interfering RNA (siRNA) interference on proliferation of SGC7901 gastric cancer cells.Methods Immunohistochemistry was used to detect the MMP-6 protein expression in 90 cases of gastric adenocarcinoma cancerous tissues and normal gastric mucosa tissues adjacent to carcinoma,and the correlation between the MMP-6 protein expression and the pathological features was analyzed.After transfection of SGC7901 cells with MMP-6 siRNA,the proliferation and cell cycle were examined by flow cytometry and methyl thiazol tetrazolium (MTT) method.Results The positive expression rate of MMP-6 protein in gastric carcinoma tissue (67.9％,62/90) was higher than in normal tissue adjacent to carcinoma (27.1％,24/90; x2 =14.812,P＜0.05).The expression of MMP-6 in gastric adenocarcinoma tissues was closely associated with lymph node metastasis and TNM stages (x2 =5.412,all P ＜0.05).Transfection of MMP-6 siRNA could inhibit the proliferation of SGC7901 cells,increase the G1 phase cells,and decrease S phase cells (F =14.459,11.813,P ＜ 0.05).Conclusion The growth and development of gastric carcinoma may be associated with the participation of MMP-6.
