中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2013年
17期
60-65
,共6页
陈芳%邓雁如%许妍妍%夏娜%高璎
陳芳%鄧雁如%許妍妍%夏娜%高瓔
진방%산안여%허연연%하나%고영
金芪降糖片%黄连%木兰花碱%表小檗碱%黄连碱%盐酸药根碱%盐酸巴马汀%盐酸小檗碱
金芪降糖片%黃連%木蘭花堿%錶小檗堿%黃連堿%鹽痠藥根堿%鹽痠巴馬汀%鹽痠小檗堿
금기강당편%황련%목란화감%표소벽감%황련감%염산약근감%염산파마정%염산소벽감
Jinqi Jiangtang Tablets%Coptidis Rhizoma%magnoflorine%epiberberine%coptisine%jatronhizine%palmatine%berberine
目的:建立HPLC同时测定黄连及金芪降糖片中木兰花碱、表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱6种生物碱含量的方法.方法:Waters Symmetry ShieldTM RP18色谱柱(4.6mm×250mm,5μm),流动相乙腈(0.3％甲酸和0.3％三乙胺)(A)-水(0.3％甲酸和0.3％三乙胺)(B)梯度洗脱,体积流量1.0 mL· min-1,检测波长345,270 nm,柱温25℃.结果:木兰花碱、表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀及盐酸小檗碱线性范围分别为0.012 06 ～0.241 2,0.038 24 ～0.7648,0.088 02 ～1.760 4,0.016 55～0.331 0,0.036 08～0.721 6,0.189 41 ～3.788 2μg(r≥0.999 8),平均加样回收率95％～105％.结论:方法专属性强、灵敏度高、重复性好、简便易行,可用于同时测定黄连及金芪降糖片中木兰花碱、表小檗碱、黄连碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱的含量,可为金芪降糖片的现代制剂研究和质量控制提供依据.
目的:建立HPLC同時測定黃連及金芪降糖片中木蘭花堿、錶小檗堿、黃連堿、鹽痠藥根堿、鹽痠巴馬汀、鹽痠小檗堿6種生物堿含量的方法.方法:Waters Symmetry ShieldTM RP18色譜柱(4.6mm×250mm,5μm),流動相乙腈(0.3％甲痠和0.3％三乙胺)(A)-水(0.3％甲痠和0.3％三乙胺)(B)梯度洗脫,體積流量1.0 mL· min-1,檢測波長345,270 nm,柱溫25℃.結果:木蘭花堿、錶小檗堿、黃連堿、鹽痠藥根堿、鹽痠巴馬汀及鹽痠小檗堿線性範圍分彆為0.012 06 ～0.241 2,0.038 24 ～0.7648,0.088 02 ～1.760 4,0.016 55～0.331 0,0.036 08～0.721 6,0.189 41 ～3.788 2μg(r≥0.999 8),平均加樣迴收率95％～105％.結論:方法專屬性彊、靈敏度高、重複性好、簡便易行,可用于同時測定黃連及金芪降糖片中木蘭花堿、錶小檗堿、黃連堿、鹽痠藥根堿、鹽痠巴馬汀、鹽痠小檗堿的含量,可為金芪降糖片的現代製劑研究和質量控製提供依據.
목적:건립HPLC동시측정황련급금기강당편중목란화감、표소벽감、황련감、염산약근감、염산파마정、염산소벽감6충생물감함량적방법.방법:Waters Symmetry ShieldTM RP18색보주(4.6mm×250mm,5μm),류동상을정(0.3％갑산화0.3％삼을알)(A)-수(0.3％갑산화0.3％삼을알)(B)제도세탈,체적류량1.0 mL· min-1,검측파장345,270 nm,주온25℃.결과:목란화감、표소벽감、황련감、염산약근감、염산파마정급염산소벽감선성범위분별위0.012 06 ～0.241 2,0.038 24 ～0.7648,0.088 02 ～1.760 4,0.016 55～0.331 0,0.036 08～0.721 6,0.189 41 ～3.788 2μg(r≥0.999 8),평균가양회수솔95％～105％.결론:방법전속성강、령민도고、중복성호、간편역행,가용우동시측정황련급금기강당편중목란화감、표소벽감、황련감、염산약근감、염산파마정、염산소벽감적함량,가위금기강당편적현대제제연구화질량공제제공의거.
