CAJ | 학술논문

目的：探讨血管紧张素Ⅱ1型受体（AT1R）拮抗剂坎地沙坦对人乳腺癌细胞系 MCF-7的生长抑制作用，为AT1R拮抗剂在乳腺癌中的应用提供实验依据。方法：噻唑蓝（MTT）比色法检测坎地沙坦对乳腺癌胞MCF-7细胞的生长抑制率，计算半数抑制浓度（IC50）。流式细胞仪分析坎地沙坦对MCF-7细胞周期和凋亡的影响。Western blot检测经坎地沙坦处理前后MCF-7细胞中野生型p53、survivin的蛋白表达差异。结果：坎地沙坦能够显著抑制乳腺癌细胞 MCF-7的增殖，使细胞凋亡显著增强，促使细胞发生 G1期周期阻滞，减小了S期细胞比例，上调其野生型p53和下调survivin蛋白表达水平。结论：坎地沙坦在体外抑制MCF-7细胞生长，诱导细胞发生周期阻滞和凋亡，机制可能与其调节p53、survivin蛋白的表达有关。
목적：탐토혈관긴장소Ⅱ1형수체（AT1R）길항제감지사탄대인유선암세포계 MCF-7적생장억제작용，위AT1R길항제재유선암중적응용제공실험의거。방법：새서람（MTT）비색법검측감지사탄대유선암포MCF-7세포적생장억제솔，계산반수억제농도（IC50）。류식세포의분석감지사탄대MCF-7세포주기화조망적영향。Western blot검측경감지사탄처리전후MCF-7세포중야생형p53、survivin적단백표체차이。결과：감지사탄능구현저억제유선암세포 MCF-7적증식，사세포조망현저증강，촉사세포발생 G1기주기조체，감소료S기세포비례，상조기야생형p53화하조survivin단백표체수평。결론：감지사탄재체외억제MCF-7세포생장，유도세포발생주기조체화조망，궤제가능여기조절p53、survivin단백적표체유관。
Objective To investigate the proliferation inhibition of angiotensin Ⅱ 1 receptor (AT1R) antagonist candesartan on human breast cancer cell line MCF-7. Methods MCF-7 cells were tested in vitro for cytotoxicity of candesartan with MTT method. The effect of candesartan on cell cycle and apoptosis of MCF-7 cells were evaluated by flow cytometry. Western Blot was applied to test the expression level of wild-type p53 and Survivin protein. Results After candesartan treatment, a dose- and time-dependent decreased proliferation in MCF-7 cells was observed. Induction of G1 cell cycle arrest and increased apoptosis in MCF-7 cells were also observed.Western blot assay showed that candesartan up-regulated wild-type P53 expression and down-regulated the Survivin expression in MCF-7 cells. Conclusions This study suggests that candesartan could inhibit the proliferation , induce G1 cell cycle arrest and apoptosis of MCF-7cellsby regulating the protein expression level of gene p53 and Survivin.