CAJ | 학술논문

通过琼脂平板法测定了牛红细胞抗菌肽SP-18对金黄色葡萄球菌、大肠杆菌的抑菌活性,以微量肉汤稀释法测定其对大肠杆菌、金黄色葡萄球菌的最小抑菌浓度（MIC）,并在扫描电镜（SEM）下观察其对细菌生物膜的影响.结果表明：SP-18对金黄色葡萄球菌、大肠杆菌有明显的抑制活性,MIC分别为1.0 mg/mL、0.25 mg/mL；SEM观察发现抗菌肽作用后细菌生物膜发生皱缩、破裂,金黄色葡萄球菌发生膜裂解,大肠杆菌表面出现孔洞结构.研究表明SP-18具有良好的抗菌活性,并通过破坏细菌生物结构的作用机制发挥抑菌效果,在抗菌肽未来的开发应用中具有良好的前景.
통과경지평판법측정료우홍세포항균태SP-18대금황색포도구균、대장간균적억균활성,이미량육탕희석법측정기대대장간균、금황색포도구균적최소억균농도（MIC）,병재소묘전경（SEM）하관찰기대세균생물막적영향.결과표명：SP-18대금황색포도구균、대장간균유명현적억제활성,MIC분별위1.0 mg/mL、0.25 mg/mL；SEM관찰발현항균태작용후세균생물막발생추축、파렬,금황색포도구균발생막렬해,대장간균표면출현공동결구.연구표명SP-18구유량호적항균활성,병통과파배세균생물결구적작용궤제발휘억균효과,재항균태미래적개발응용중구유량호적전경.
The bioactivity of novel bovine red blood cell antimicrobial peptide SP-18 to Staphylococcus aureus, Escherichia coli was tested by agar gel diffusion method in this paper.The minimal inhibitory concentration of bovine red blood cell antimicrobial peptide to S. aureus,E. coli was tested by broth micro-dilution method.The morphology of bacterias was observed by scanning electron microscope.The results showed that SP-18 have inhibitory action to tested bacteria,the MIC to S. aureus and E. coli were 1.0 mg/mL and 0.25 mg/mL, respectively.The cell membrane of tested bacteria were observed shrinking,distorting.The cell membrane of S. aureus was cracked.The surface of E. coil has many holes.These results indicated red blood cell antimicrobial peptide restrained the bacteria by damaging its cell membrane,which had a potential advantage in the development and application.