CAJ | 학술논문

建立了整体柱固相萃取-高效液相色谱/紫外检测在线联用方法并用于水果中氯吡脲的分析测定。考察了萃取溶剂的 pH 值及其流速、溶剂解吸时间及其流速等因素对氯吡脲萃取率的影响。在优化的条件下，该方法对氯吡脲的富集倍数为214倍，方法的线性范围为0.01~50.00μg / L，检出限为25 ng / L，相对标准偏差（ RSD）为3.9％。黑葡萄、猕猴桃和西瓜等水果中氯吡脲的加标回收率为87.0％~120.7％，RSD 为0.6％~9.2％。结果表明该方法快速、灵敏，能满足水果中痕量氯吡脲残留分析的要求。
건립료정체주고상췌취-고효액상색보/자외검측재선련용방법병용우수과중록필뇨적분석측정。고찰료췌취용제적 pH 치급기류속、용제해흡시간급기류속등인소대록필뇨췌취솔적영향。재우화적조건하，해방법대록필뇨적부집배수위214배，방법적선성범위위0.01~50.00μg / L，검출한위25 ng / L，상대표준편차（ RSD）위3.9％。흑포도、미후도화서과등수과중록필뇨적가표회수솔위87.0％~120.7％，RSD 위0.6％~9.2％。결과표명해방법쾌속、령민，능만족수과중흔량록필뇨잔류분석적요구。
A novel method based on the monolithic column solid-phase extraction(SPE)cou-pled with high performance liquid chromatography in online mode was developed for the deter-mination of trace forchlorfenuron in fruits. The enrichment factor of SPE for forchlorfenuron was 214. The method was fully validated. It showed a linear range of 0. 01-50. 00 μg / L and a limit of detection of 25 ng / L,with the relative standard deviation(RSD)of 3. 9% . The proposed method was successfully applied for the determination of forchlorfenuron in grape,kiwifruit and watermelon samples,with the recoveries of 87. 0% -120. 7% and the RSDs of 0. 6% -9. 2% . This online method was proved to be selective,sensitive and convenient for the determination of trace forchlorfenuron in complex samples.