CAJ | 학술논문

目的：利用光谱学技术研究卵巢癌患者的血清自体荧光光谱特点。方法：应用荧光分光光度计，使用350 nm激发光，检测了不同放置时间标本的荧光光谱特点；测定了不同波长激发光条件下37例卵巢癌患者和40例正常者的血清荧光光谱，并对300 nm激发光光谱的峰位、峰高、峰面积进行了分析。结果：血清标本放置6 h测得的荧光光谱峰的位置、峰强度、峰面积等特点较为显著。300 nm激发光形成的荧光光谱在正常者与卵巢癌患者之间具有最大的差异。卵巢癌患者自身荧光光谱的峰位紫移；峰高及峰面积均高于正常者。结论：通过检测血清自体荧光光谱的特征性改变可能对卵巢癌患者的早期诊断具有重要临床意义。
목적：이용광보학기술연구란소암환자적혈청자체형광광보특점。방법：응용형광분광광도계，사용350 nm격발광，검측료불동방치시간표본적형광광보특점；측정료불동파장격발광조건하37례란소암환자화40례정상자적혈청형광광보，병대300 nm격발광광보적봉위、봉고、봉면적진행료분석。결과：혈청표본방치6 h측득적형광광보봉적위치、봉강도、봉면적등특점교위현저。300 nm격발광형성적형광광보재정상자여란소암환자지간구유최대적차이。란소암환자자신형광광보적봉위자이；봉고급봉면적균고우정상자。결론：통과검측혈청자체형광광보적특정성개변가능대란소암환자적조기진단구유중요림상의의。
Objective:Using the technique of spectrum investigate the characteristics of the serum auto-fluorescent spectrum of patients with ovarian cancer. Methods:Using fluorospectrophotometer and 350 nm excitation light ,the present study tested the serum auto-fluorescent spectrum of samples that been kept for different durations. It was also studied that the serum auto-fluorescent spectrums of 40 normal women and 37 patients with ovarian cancer ,using different wavelength of excitation lights,and the data were further analyzed and compared including peak positions,peak heights and peak areas of spectrums,respectively. Results:The most prominent changes in peak position,peak height and peak area were observed in spectrum of sample that placed for 6 hrs. Difference in serum auto-fluorescent spectrum between ovarian patients and normal women were found to be most prominent under the 300 nm excitation light. Compared with those of normal controls ,serum atuo-fluroesent spectrums in patients with ovarian cancer had purple shift of peak position ,higher peak height,and larger peak area. Conclusions:There is probably clinical significance of detecting the serum auto-fluorescent spectrum in early diagnosis of ovarian cancer.