中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2014年
18期
5-6,7
,共3页
卢志权%谢一鸣%成光辉%李介华
盧誌權%謝一鳴%成光輝%李介華
로지권%사일명%성광휘%리개화
结直肠肿瘤%胸苷激酶1%糖类抗原199%糖类抗原724%联合检测
結直腸腫瘤%胸苷激酶1%糖類抗原199%糖類抗原724%聯閤檢測
결직장종류%흉감격매1%당류항원199%당류항원724%연합검측
Colorectal neoplasms%Serum thymidine kinase 1%Carbohydrate antigen 199%Carbohydrate antigen 724%Combined detection
目的：探讨血清胸苷激酶1(TK1)、糖类抗原199(CA199)、糖类抗原724(CA724)联合检测对结直肠癌诊断的临床应用价值。方法采用电化学发光法对77例结直肠癌患者、61例结直肠良性病变患者和40例健康体检者的血清CA199、CA724含量进行检测，采用免疫印迹-增强化学发光法对各组血清TK1含量进行检测。结果结直肠癌组的TK1、CA199和CA724水平显著高于结直肠良性病变组患者和正常对照组(P均<0.05)，结直肠良性病变患者的血清TK1、CA199和CA724水平显著高于正常对照组(P<0.05)。单项检测TK1对结直肠癌诊断的敏感度、特异度和准确度分别为40.3%、75.4%、55.8%， CA199为44.2%、88.5%、63.8%， CA724为45.5%、77.0%、59.4%；联合检测TK1、CA199和CA724为64.9%、68.9%、65.9%。结论 TK1、CA199和CA724水平对于结直肠癌的诊断、鉴别诊断具有重要的临床价值，三者联合检测可提高结直肠癌的检出率。
目的：探討血清胸苷激酶1(TK1)、糖類抗原199(CA199)、糖類抗原724(CA724)聯閤檢測對結直腸癌診斷的臨床應用價值。方法採用電化學髮光法對77例結直腸癌患者、61例結直腸良性病變患者和40例健康體檢者的血清CA199、CA724含量進行檢測，採用免疫印跡-增彊化學髮光法對各組血清TK1含量進行檢測。結果結直腸癌組的TK1、CA199和CA724水平顯著高于結直腸良性病變組患者和正常對照組(P均<0.05)，結直腸良性病變患者的血清TK1、CA199和CA724水平顯著高于正常對照組(P<0.05)。單項檢測TK1對結直腸癌診斷的敏感度、特異度和準確度分彆為40.3%、75.4%、55.8%， CA199為44.2%、88.5%、63.8%， CA724為45.5%、77.0%、59.4%；聯閤檢測TK1、CA199和CA724為64.9%、68.9%、65.9%。結論 TK1、CA199和CA724水平對于結直腸癌的診斷、鑒彆診斷具有重要的臨床價值，三者聯閤檢測可提高結直腸癌的檢齣率。
목적：탐토혈청흉감격매1(TK1)、당류항원199(CA199)、당류항원724(CA724)연합검측대결직장암진단적림상응용개치。방법채용전화학발광법대77례결직장암환자、61례결직장량성병변환자화40례건강체검자적혈청CA199、CA724함량진행검측，채용면역인적-증강화학발광법대각조혈청TK1함량진행검측。결과결직장암조적TK1、CA199화CA724수평현저고우결직장량성병변조환자화정상대조조(P균<0.05)，결직장량성병변환자적혈청TK1、CA199화CA724수평현저고우정상대조조(P<0.05)。단항검측TK1대결직장암진단적민감도、특이도화준학도분별위40.3%、75.4%、55.8%， CA199위44.2%、88.5%、63.8%， CA724위45.5%、77.0%、59.4%；연합검측TK1、CA199화CA724위64.9%、68.9%、65.9%。결론 TK1、CA199화CA724수평대우결직장암적진단、감별진단구유중요적림상개치，삼자연합검측가제고결직장암적검출솔。
Objective To investigate diagnostic value of combined detection of TK1, CA199 and CA724 in colorectal cancer. Methods Serum levels of CAl99 and CA724 in 77 patients with colorectal cancer, 61 patients with benign colorectal diseases and 40 healthy controls were measured by western blotting-enhanced chemiluminescence method, and serum levels of TK1 were measured by enzyme immune chemiluminescence dot-blot assay. Results Serum levels of CAl99 and CA724 in patients with colorectal cancer were significantly higher than those in patients with benign colorectal diseases and healthy controls(P<0.05). And those patients with benign colorectal diseases were significantly higher than those in healthy controls(P<0.05). The sensitivity, specificity and accuracy of TK1 for the diagnosis of colorectal cancer were 40.3%, 75.4%and 55.8%, respectively;those of CA199 were 44.2%, 88.5%and 63.8%, respectively;CA724 were 45.5%,77.0%and 59.4%, respectively;combined detection of TK1, CA199 and CA724 were 64.9%, 68.9% and 65.9%, respectively. Conclusion There are certain values of combined detection of TK1, CA199 and CA724 for the diagnosis of colorectal cancer, combined detection of TK1, CA199 and CA724 were helpful to increase the detection rate of colorectal cancer.
