CAJ | 학술논문

目的：检测 Attractin (Atrn)mRNA和 Atrn蛋白在小鼠睾丸组织中的表达以及 Atrn蛋白在睾丸组织的分布.方法：取10~12周龄雄性BALB/c 小鼠睾丸组织,应用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和Western blot方法,检测 Atrn mRNA和 Atrn蛋白在成熟雄性小鼠睾丸组织中的表达,用免疫组织化学方法检测 Atrn蛋白在睾丸组织的分布.结果：RT-PCR扩增产物经琼脂糖凝胶电泳,显示为单一、特异扩增条带,与预期产物片断大小一致. Western Blot 检测到 Atrn蛋白,分子量约为160KD.免疫荧光组织化学结果显示 Atrn蛋白在小鼠睾丸组织分布广泛,表达于精原细胞、精母细胞、精子细胞、Sertoli细胞、Leydig细胞和管周肌样细胞,主要表达于胞膜.结论：成熟小鼠睾丸组织表达 Atrn mRNA和 Atrn蛋白.Atrn蛋白定位于间质细胞和生精细胞胞膜.
목적：검측 Attractin (Atrn)mRNA화 Atrn단백재소서고환조직중적표체이급 Atrn단백재고환조직적분포.방법：취10~12주령웅성BALB/c 소서고환조직,응용역전록-취합매련반응(reverse transcription-polymerase chain reaction,RT-PCR)화Western blot방법,검측 Atrn mRNA화 Atrn단백재성숙웅성소서고환조직중적표체,용면역조직화학방법검측 Atrn단백재고환조직적분포.결과：RT-PCR확증산물경경지당응효전영,현시위단일、특이확증조대,여예기산물편단대소일치. Western Blot 검측도 Atrn단백,분자량약위160KD.면역형광조직화학결과현시 Atrn단백재소서고환조직분포엄범,표체우정원세포、정모세포、정자세포、Sertoli세포、Leydig세포화관주기양세포,주요표체우포막.결론：성숙소서고환조직표체 Atrn mRNA화 Atrn단백.Atrn단백정위우간질세포화생정세포포막.
Objective:To examine the expression of Attractin(Atrn)mRNA and Attractin protein in the mouse testis.And then investigate the distribution of Atrn protein in the mouse testis.Methods:Testis from 10~12weeks old BALB/c mice were used for the study of Atrn expression and distribution by RT-PCR, Western blot and immunofluorescence.Results:The Atrn mRNA and Atrn protein were both expressed in mouse testis.In the testis,distinct immunopositive staining for Atrn protein was detected on cell membrane within spermatogonia,primary spermatocyte,spermatid,Sertoli cell,Leydig cell and peritubular myoid cells.Conclusion:Atrn is expressed in the Leydig cells and germ cells,suggesting that it may play important roles in male reproductive physiological processes.