东南大学学报（医学版）
東南大學學報（醫學版）
동남대학학보（의학판）
JOURNAL OF SOUTHEAST UNIVERSITY(MEDICAL SCIENCE EDITION)
2014年
2期
147-150
,共4页
刘莉%刘慧敏%顾海伦%赵越%杨军
劉莉%劉慧敏%顧海倫%趙越%楊軍
류리%류혜민%고해륜%조월%양군
邻苯二甲酸二己酯%脂肪分布%磷脂酰肌醇蛋白聚糖-4%Tbox-15%小鼠
鄰苯二甲痠二己酯%脂肪分佈%燐脂酰肌醇蛋白聚糖-4%Tbox-15%小鼠
린분이갑산이기지%지방분포%린지선기순단백취당-4%Tbox-15%소서
di-2-ethylhexylphthalate%fat distribution%glypican 4%Tbox-15%mice
目的：探讨孕鼠暴露低剂量邻苯二甲酸二己酯（ di-2-ethylhexylphthalate ，DEHP）对仔鼠脂肪分布的影响及机制。方法：将C57BL/6J孕鼠分为2组，对照组喂饲基础饲料，DEHP组喂饲添加质量分数为0.06％DEHP的基础饲料，至仔鼠出生。9周后，处死仔鼠，切取附睾周围和腹股沟皮下脂肪组织，称质量；实时定量RT-PCR法检测Gpc4、Tbx15 mRNA表达。结果：DEHP组仔鼠附睾脂肪组织质量显著高于对照组（ P＜0.05）；对照组和DEHP组仔鼠附睾脂肪组织Gpc4 mRNA表达均显著高于皮下脂肪组织（ P＜0.05），而Tbx15 mRNA表达则显著低于皮下脂肪组织（ P＜0.01）；相比对照组， DEHP组仔鼠附睾脂肪组织 Gpc4 mRNA及皮下脂肪组织Tbx15 mRNA表达均显著增加（ P＜0.01）。结论：胚胎期低剂量DEHP暴露可能通过改变内脏及皮下Gpc4和Tbx15的表达，影响脂肪组织的分布。
目的：探討孕鼠暴露低劑量鄰苯二甲痠二己酯（ di-2-ethylhexylphthalate ，DEHP）對仔鼠脂肪分佈的影響及機製。方法：將C57BL/6J孕鼠分為2組，對照組餵飼基礎飼料，DEHP組餵飼添加質量分數為0.06％DEHP的基礎飼料，至仔鼠齣生。9週後，處死仔鼠，切取附睪週圍和腹股溝皮下脂肪組織，稱質量；實時定量RT-PCR法檢測Gpc4、Tbx15 mRNA錶達。結果：DEHP組仔鼠附睪脂肪組織質量顯著高于對照組（ P＜0.05）；對照組和DEHP組仔鼠附睪脂肪組織Gpc4 mRNA錶達均顯著高于皮下脂肪組織（ P＜0.05），而Tbx15 mRNA錶達則顯著低于皮下脂肪組織（ P＜0.01）；相比對照組， DEHP組仔鼠附睪脂肪組織 Gpc4 mRNA及皮下脂肪組織Tbx15 mRNA錶達均顯著增加（ P＜0.01）。結論：胚胎期低劑量DEHP暴露可能通過改變內髒及皮下Gpc4和Tbx15的錶達，影響脂肪組織的分佈。
목적：탐토잉서폭로저제량린분이갑산이기지（ di-2-ethylhexylphthalate ，DEHP）대자서지방분포적영향급궤제。방법：장C57BL/6J잉서분위2조，대조조위사기출사료，DEHP조위사첨가질량분수위0.06％DEHP적기출사료，지자서출생。9주후，처사자서，절취부고주위화복고구피하지방조직，칭질량；실시정량RT-PCR법검측Gpc4、Tbx15 mRNA표체。결과：DEHP조자서부고지방조직질량현저고우대조조（ P＜0.05）；대조조화DEHP조자서부고지방조직Gpc4 mRNA표체균현저고우피하지방조직（ P＜0.05），이Tbx15 mRNA표체칙현저저우피하지방조직（ P＜0.01）；상비대조조， DEHP조자서부고지방조직 Gpc4 mRNA급피하지방조직Tbx15 mRNA표체균현저증가（ P＜0.01）。결론：배태기저제량DEHP폭로가능통과개변내장급피하Gpc4화Tbx15적표체，영향지방조직적분포。
Objective: To investigate the effect and mechanism of low dose di-2-ethylhexylphthalate ( DEHP ) exposure on fat distribution of offspring in pregnant mice .Methods: Pregnant C57BL/6J mice were divided into two groups.Mice in control group were fed with chow diet , and mice in DEHP group were fed with chow diet supplemented with 0.06%( w/w ) DEHP till their offspring was born .Nine weeks after birth , the mice were sacrificed .Epididymal and inguinal subcutaneous adipose tissues were dissected and weighted .The expression of Gpc4 and Tbx15 mRNA was measured by real-time reverse transcription polymerase chain reaction ( RT-PCR ) method.Results:The weight of epididymal adipose tissues in DEHP group was significantly higher than that in control group (P<0.05).The expression of Gpc4 mRNA in epididymal adipose tissues was significantly higher than that in subcutaneous adipose tissues ( P<0.05 ) , while the expression of Tbx 15 mRNA was significantly lower than that in subcutaneous adipose tissues ( P<0.01 ) in both control and DEHP group .Compared with control group , Gpc4 mRNA expression in epididymal adipose tissues and Tbx 15 mRNA expression in subcutaneous adipose tissues were increased ( P<0.01) .Conclusion:Low dose DEHP exposure in mice prenatal period perhaps affects fat distribution via modifying the expression of Gpc 4 and Tbx15 mRNA in visceral and subcutaneous adipose tissues.
