干旱地区农业研究
榦旱地區農業研究
간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2014年
2期
157-162
,共6页
深绿木霉%促生作用%黑麦草%生理生化特性
深綠木黴%促生作用%黑麥草%生理生化特性
심록목매%촉생작용%흑맥초%생리생화특성
Trichoderma aureoviride%growth promoting%ryegrass%physiological characteristics
通过种子萌发和幼苗盆栽试验研究了不同稀释倍数深绿木霉发酵液对黑麦草促生作用及生理生化特性的影响。室内试验结果表明，不同稀释倍数深绿木霉发酵液能够显著提高黑麦草种子的发芽率、发芽指数和活力指数，尤其是100倍稀释液对黑麦草种子发芽率、发芽指数和活力指数的影响较为明显，分别为96．55％、37．41和7．83。盆栽试验结果表明，不同稀释倍数深绿木霉发酵液对黑麦草幼苗的生长具有明显的促生作用，以及能够显著地提高幼苗叶绿素、可溶性蛋白含量和与抗性相关酶的活性，尤其是100倍稀释液处理的黑麦草幼苗根系长度、株高、鲜重、干重和根冠比的相对增长率分别为16．95％、13．33％、40．57％、73．68％和36．36％；叶绿素和可溶性蛋白含量的相对增长率分别为84．09％和75．50％；多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶活性均显著高于对照，且多酚氧化酶和苯丙氨酸解氨酶活性在处理后第四天达到最大值，过氧化物酶活性在处理后第三天达到最大值。因此，深绿木霉发酵液对黑麦草的生长具有显著的促生作用，且能够显著提高与抗性相关酶的活性。
通過種子萌髮和幼苗盆栽試驗研究瞭不同稀釋倍數深綠木黴髮酵液對黑麥草促生作用及生理生化特性的影響。室內試驗結果錶明，不同稀釋倍數深綠木黴髮酵液能夠顯著提高黑麥草種子的髮芽率、髮芽指數和活力指數，尤其是100倍稀釋液對黑麥草種子髮芽率、髮芽指數和活力指數的影響較為明顯，分彆為96．55％、37．41和7．83。盆栽試驗結果錶明，不同稀釋倍數深綠木黴髮酵液對黑麥草幼苗的生長具有明顯的促生作用，以及能夠顯著地提高幼苗葉綠素、可溶性蛋白含量和與抗性相關酶的活性，尤其是100倍稀釋液處理的黑麥草幼苗根繫長度、株高、鮮重、榦重和根冠比的相對增長率分彆為16．95％、13．33％、40．57％、73．68％和36．36％；葉綠素和可溶性蛋白含量的相對增長率分彆為84．09％和75．50％；多酚氧化酶、過氧化物酶和苯丙氨痠解氨酶活性均顯著高于對照，且多酚氧化酶和苯丙氨痠解氨酶活性在處理後第四天達到最大值，過氧化物酶活性在處理後第三天達到最大值。因此，深綠木黴髮酵液對黑麥草的生長具有顯著的促生作用，且能夠顯著提高與抗性相關酶的活性。
통과충자맹발화유묘분재시험연구료불동희석배수심록목매발효액대흑맥초촉생작용급생리생화특성적영향。실내시험결과표명，불동희석배수심록목매발효액능구현저제고흑맥초충자적발아솔、발아지수화활력지수，우기시100배희석액대흑맥초충자발아솔、발아지수화활력지수적영향교위명현，분별위96．55％、37．41화7．83。분재시험결과표명，불동희석배수심록목매발효액대흑맥초유묘적생장구유명현적촉생작용，이급능구현저지제고유묘협록소、가용성단백함량화여항성상관매적활성，우기시100배희석액처리적흑맥초유묘근계장도、주고、선중、간중화근관비적상대증장솔분별위16．95％、13．33％、40．57％、73．68％화36．36％；협록소화가용성단백함량적상대증장솔분별위84．09％화75．50％；다분양화매、과양화물매화분병안산해안매활성균현저고우대조，차다분양화매화분병안산해안매활성재처리후제사천체도최대치，과양화물매활성재처리후제삼천체도최대치。인차，심록목매발효액대흑맥초적생장구유현저적촉생작용，차능구현저제고여항성상관매적활성。
Effects of different dilutions of the fermentation medium of Trichoderma aureoviride on growth and physio-logical characteristics of ryegrass were studied by seed germination and seedling pot experiment in vitro and pot experi -ments .Results showed that different dilutions of the fermentation medium of T .aureoviride can significantly increase the rates of seed germination ,germination index and vigor index ,especially for the dilutions of 100 times by which seed ger-mination rates ,germination index and vigor index were increased by 96 .55% ,37 .41 and 7 .83 ,respectively .Pot exper-iment results showed that different dilutions of the fermentation medium of T . aureoviride had obvious effects on the growth of ryegrass seedlings ,and can significantly increase the content of chlorophyll ,protein ,and defensive enzymes . Especially the dilutions of 100 times can increase ryegrass root length ,plant height ,fresh weight and dry weight by 16 .95% 、13 .33% 、40 .57% 、73 .68% ,and 36 .36% ,respectively .The content of chlorophyll and soluble protein were increased by 84 .09% and 75 .50% .Polyphenol oxidase ,peroxidase and phenylalanine enzyme activity were significantly higher than control after treatment with the dilutions of 100 times of T . aureoviride .Maximum activities of POD ,PPO and PAL were observed on 3 and 4 days after inoculation .Therefore ,the results suggested that the fermentation medium of T . aureoviride has substantial effects on growth and physiological characteristics of ryegrass .
