中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2013年
18期
155-157
,共3页
易荆丽%张嘉家%周毅生%赵永恒%王嵩
易荊麗%張嘉傢%週毅生%趙永恆%王嵩
역형려%장가가%주의생%조영항%왕숭
高效液相色谱%龙须藤%多甲氧基黄酮%含量测定
高效液相色譜%龍鬚籐%多甲氧基黃酮%含量測定
고효액상색보%룡수등%다갑양기황동%함량측정
HPLC%Bauhinia championii%polymethoxylated flavonoids%quantitative determination
目的:建立HPLC测定龙须藤中5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基含量的方法.方法:DIKMA PlatisilODS C18色谱柱(4.6 mm ×250 mm,5μm),流动相乙腈(A)-0.1％磷酸溶液(B),梯度洗脱(0 min,25％ A;15 min,45％A；40 min,50％ A),流速0.8 mL· min-,检测波长324 nm.结果:5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3',4'-亚甲二氧基分别在0.0084～0.3360 μg(r =0.999 9),0.013 4～0.536 0 μg(r =0.999 7)与峰面积呈良好的线性关系,平均回收率分别为99.41％,98.65％,RSD分别为0.57％,1.01％.结论:该方法操作简单准确,分离效果、精密度和重复性好,适合于测定该药材中5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基的含量.
目的:建立HPLC測定龍鬚籐中5,7,3’,4’,5’-五甲氧基黃酮和5,6,7,5’-四甲氧基-3’,4’-亞甲二氧基含量的方法.方法:DIKMA PlatisilODS C18色譜柱(4.6 mm ×250 mm,5μm),流動相乙腈(A)-0.1％燐痠溶液(B),梯度洗脫(0 min,25％ A;15 min,45％A；40 min,50％ A),流速0.8 mL· min-,檢測波長324 nm.結果:5,7,3’,4’,5’-五甲氧基黃酮和5,6,7,5’-四甲氧基-3',4'-亞甲二氧基分彆在0.0084～0.3360 μg(r =0.999 9),0.013 4～0.536 0 μg(r =0.999 7)與峰麵積呈良好的線性關繫,平均迴收率分彆為99.41％,98.65％,RSD分彆為0.57％,1.01％.結論:該方法操作簡單準確,分離效果、精密度和重複性好,適閤于測定該藥材中5,7,3’,4’,5’-五甲氧基黃酮和5,6,7,5’-四甲氧基-3’,4’-亞甲二氧基的含量.
목적:건립HPLC측정룡수등중5,7,3’,4’,5’-오갑양기황동화5,6,7,5’-사갑양기-3’,4’-아갑이양기함량적방법.방법:DIKMA PlatisilODS C18색보주(4.6 mm ×250 mm,5μm),류동상을정(A)-0.1％린산용액(B),제도세탈(0 min,25％ A;15 min,45％A；40 min,50％ A),류속0.8 mL· min-,검측파장324 nm.결과:5,7,3’,4’,5’-오갑양기황동화5,6,7,5’-사갑양기-3',4'-아갑이양기분별재0.0084～0.3360 μg(r =0.999 9),0.013 4～0.536 0 μg(r =0.999 7)여봉면적정량호적선성관계,평균회수솔분별위99.41％,98.65％,RSD분별위0.57％,1.01％.결론:해방법조작간단준학,분리효과、정밀도화중복성호,괄합우측정해약재중5,7,3’,4’,5’-오갑양기황동화5,6,7,5’-사갑양기-3’,4’-아갑이양기적함량.
