中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2013年
8期
537-541
,共5页
周将%廖蕴华%薛超%伍巧源%潘玲%卜昆鹏%彭茜%叶旭东%韦晓梨
週將%廖蘊華%薛超%伍巧源%潘玲%蔔昆鵬%彭茜%葉旭東%韋曉梨
주장%료온화%설초%오교원%반령%복곤붕%팽천%협욱동%위효리
抗体,抗中性白细胞胞质%血管炎%DNA甲基转移酶%CD11a基因%外周血单个核细胞
抗體,抗中性白細胞胞質%血管炎%DNA甲基轉移酶%CD11a基因%外週血單箇覈細胞
항체,항중성백세포포질%혈관염%DNA갑기전이매%CD11a기인%외주혈단개핵세포
Antibodies,antineutrophil cytoplasmic%Vasculitis%DNA methyltransferase%CD11a%Peripheral blood mononuclear cells
目的 探讨DNA甲基转移酶1(DNMT1)、甲基转移酶3a(DNMT3a)、甲基转移酶3b(DNMT3b)与CD11a的相互关系,以及与中国广西人群中原发性抗中性粒细胞胞质抗体(ANCA)相关性小血管炎(AAV)发病的相关关系.方法 采用荧光定量-聚合酶链反应(qRT-PCR)技术检测3种甲基转移酶以及CD1 1a的mRNA在33例广西活动期AAV患者和45名健康对照组外周血单个核细胞中的表达,采用t检验及Spearman、Pearson进行相关分析.结果 ①AAV患者组的DNMT1的mRNA表达水平0.5±0.3明显低于健康对照组0.8±0.3,差异有统计学意义(t=4.446,P＜0.01)；②AAV患者组的DNMT3a、DNMT3b的mRNA表达水平与健康对照组之间的差异无统计学意义(P＞0.05)；③AAV患者组的CD11a的mRNA表达水平0.63±0.46高于健康对照组0.04±0.22(t=2.402,P＜0.05)；④AAV患者中DNMT1的表达水平与患者BAVS评分呈负相关(r=-0.414,P＜0.05)；⑤AAV患者中CD11a的表达水平与BAVS评分呈正相关(r=0.435,P＜0.05)；⑥AAV患者中DNMT1与CD11a的表达水平呈负相关(r=-0.15,P＜0.05).⑦AAV患者中BAVS评分与肾脏病理活动指数(AI)呈正相关(r=0.786,P＜0.05).结论 中国广西人群中,AAV患者外周血单个核细胞中DNMT1基因表达的降低可能是造成DNA低甲基化的重要原因,可能诱导CD11a等相关致病基因过度表达从而引起AAV的发病.
目的 探討DNA甲基轉移酶1(DNMT1)、甲基轉移酶3a(DNMT3a)、甲基轉移酶3b(DNMT3b)與CD11a的相互關繫,以及與中國廣西人群中原髮性抗中性粒細胞胞質抗體(ANCA)相關性小血管炎(AAV)髮病的相關關繫.方法 採用熒光定量-聚閤酶鏈反應(qRT-PCR)技術檢測3種甲基轉移酶以及CD1 1a的mRNA在33例廣西活動期AAV患者和45名健康對照組外週血單箇覈細胞中的錶達,採用t檢驗及Spearman、Pearson進行相關分析.結果 ①AAV患者組的DNMT1的mRNA錶達水平0.5±0.3明顯低于健康對照組0.8±0.3,差異有統計學意義(t=4.446,P＜0.01)；②AAV患者組的DNMT3a、DNMT3b的mRNA錶達水平與健康對照組之間的差異無統計學意義(P＞0.05)；③AAV患者組的CD11a的mRNA錶達水平0.63±0.46高于健康對照組0.04±0.22(t=2.402,P＜0.05)；④AAV患者中DNMT1的錶達水平與患者BAVS評分呈負相關(r=-0.414,P＜0.05)；⑤AAV患者中CD11a的錶達水平與BAVS評分呈正相關(r=0.435,P＜0.05)；⑥AAV患者中DNMT1與CD11a的錶達水平呈負相關(r=-0.15,P＜0.05).⑦AAV患者中BAVS評分與腎髒病理活動指數(AI)呈正相關(r=0.786,P＜0.05).結論 中國廣西人群中,AAV患者外週血單箇覈細胞中DNMT1基因錶達的降低可能是造成DNA低甲基化的重要原因,可能誘導CD11a等相關緻病基因過度錶達從而引起AAV的髮病.
목적 탐토DNA갑기전이매1(DNMT1)、갑기전이매3a(DNMT3a)、갑기전이매3b(DNMT3b)여CD11a적상호관계,이급여중국엄서인군중원발성항중성립세포포질항체(ANCA)상관성소혈관염(AAV)발병적상관관계.방법 채용형광정량-취합매련반응(qRT-PCR)기술검측3충갑기전이매이급CD1 1a적mRNA재33례엄서활동기AAV환자화45명건강대조조외주혈단개핵세포중적표체,채용t검험급Spearman、Pearson진행상관분석.결과 ①AAV환자조적DNMT1적mRNA표체수평0.5±0.3명현저우건강대조조0.8±0.3,차이유통계학의의(t=4.446,P＜0.01)；②AAV환자조적DNMT3a、DNMT3b적mRNA표체수평여건강대조조지간적차이무통계학의의(P＞0.05)；③AAV환자조적CD11a적mRNA표체수평0.63±0.46고우건강대조조0.04±0.22(t=2.402,P＜0.05)；④AAV환자중DNMT1적표체수평여환자BAVS평분정부상관(r=-0.414,P＜0.05)；⑤AAV환자중CD11a적표체수평여BAVS평분정정상관(r=0.435,P＜0.05)；⑥AAV환자중DNMT1여CD11a적표체수평정부상관(r=-0.15,P＜0.05).⑦AAV환자중BAVS평분여신장병리활동지수(AI)정정상관(r=0.786,P＜0.05).결론 중국엄서인군중,AAV환자외주혈단개핵세포중DNMT1기인표체적강저가능시조성DNA저갑기화적중요원인,가능유도CD11a등상관치병기인과도표체종이인기AAV적발병.
Objective To investigate the relationship of DNA methyltransferase 1 (DNMT1),methyltransferase 3a (DNMT3a),Methyltransferase 3b(DNMT3b) and CD 11 a in ANCA-associated vasculitis (AAV) in Chinese Guangxi population.Methods The mRNA level of three kinds of DNMT and CD11a in the peripheral blood mononuclear cells (PBMC) from 33 cases with active AAV and 45 healthy controls were examined by fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR),the difference in gene expression was compared between the AAV groups and healthy controls.T-test was used for statistical analysis.Correlation analysis was used for gene expression analysis between the AAV groups and its clinical BVAS score and the correlation between renal pathological activity index (AI).Results ① Statistical significant difference was found in the mRNA expression of DNMT1 between the AAV group 0.5 ±0.3 and healthy controls 0.8±0.3 (t=4.446,P＜0.01).The expression of DNMT1 in PBMC was decreased significantly than that in healthy controls.② No statistical significant difference was found in the mRNA expression of DNMT3a and DNMT3b between the AAV group and healthy controls (P＞0.05).③ Statistical significant difference was found in the mRNA expression of CD11a between the AAV group 0.63±0.46 and healthy controls 0.44±0.22 (t=2.402,P＜0.05).The mRNA expression of CD11a in PBMC was higher than healthy controls.④ The mRNA expression levels of DNMT1 in PBMC of AAV patients and BVAS score showed a negative correlation (r=-0.414,P＜0.05).⑤ The mRNA expression levels of CD11a in PBMC of AAV patients and BVAS score was positively correlated (r=0.435,P＜0.05).⑥ The mRNA expression levels of DNMT1 and CD11a were negatively correlated (r=-0.15,P＜0.05).⑦ Renal pathological activity index (AI) and BVAS score of the AAV patients was positively correlated (r=0.786,P＜0.05).Conclusion The decreased expression of DNMT1 may result in the decline of DNA methylation in AAV,the hypomethylation of DNA may upregulate the CD11a transcription.The decreased expression of DNMT1 may participate in the pathogenesis of AAV.
