CAJ | 학술논문

目的探讨外源性Rb94基因联合γ射线照射对食管癌细胞株K150生长的抑制作用,为临床上进行Rb94基因.放射治疗肿瘤奠定实验基础.方法将重组腺病毒Ad-Rb94于体外转染K150细胞,转染后进行6 Gy~(137)C8 γ射线照射,数字随机表法分为空白对照组、Ad-LacZ对照组、Ad-Rb94组、照射组和Ad-Rb94联合照射组,观察K150细胞的生长曲线、细胞周期、细胞凋亡和Rb94基因表达的变化.结果 Ad-Rb94组、照射组和联合照射组对K150细胞生长均具有抑制作用,联合照射组的抑制效应最强(45.49%),明显高于Ad-Rb94组(20.26%)和照射组(35.11%)(χ~2=14.25,P<0.05).联合照射组K150细胞出现明显的G_1期阻滞和细胞凋亡,G_1期细胞和凋亡细胞所占比例最高,分别达到73.4%和15.7%.联合照射组K150细胞中Rb94基因表达量最多,分别是Ad-Rb94组和空白对照组的1.87倍和2.92倍.结论 Rb94基因联合γ射线照射对食管癌细胞的抑瘤作用具有协同效应,有效地抑制肿瘤细胞的生长.
목적 탐토외원성Rb94기인연합γ사선조사대식관암세포주K150생장적억제작용,위림상상진행Rb94기인.방사치료종류전정실험기출.방법 장중조선병독Ad-Rb94우체외전염K150세포,전염후진행6 Gy~(137)C8 γ사선조사,수자수궤표법분위공백대조조、Ad-LacZ대조조、Ad-Rb94조、조사조화Ad-Rb94연합조사조,관찰K150세포적생장곡선、세포주기、세포조망화Rb94기인표체적변화.결과 Ad-Rb94조、조사조화연합조사조대K150세포생장균구유억제작용,연합조사조적억제효응최강(45.49%),명현고우Ad-Rb94조(20.26%)화조사조(35.11%)(χ~2=14.25,P<0.05).연합조사조K150세포출현명현적G_1기조체화세포조망,G_1기세포화조망세포소점비례최고,분별체도73.4%화15.7%.연합조사조K150세포중Rb94기인표체량최다,분별시Ad-Rb94조화공백대조조적1.87배화2.92배.결론 Rb94기인연합γ사선조사대식관암세포적억류작용구유협동효응,유효지억제종류세포적생장.
Objective To study the combined effect of foreign retinoblastoma 94 gene and ionizing radiation on the growth of esophageal carcinoma cells K150. Method K150 cells was transfected by recombinant Rb94 gene adenovirus vector and irradiated by 6 Gy ~(l37)Cs γ-ray after transfection. The cohorts were divided into blank control, Ad-lacZ, Ad-Rb94, irradiation and Ad-Rb94 combined with irradiation. Cell growth, cell cycle, cell apoptosis and expression of retinoblastoma 94 gene of K150 cells were detected. Results The growth of K150 cells transfected with Ad-Rb94, radiation and Ad-Rb94 combined with irradiation group at 7 day after infection was all inhibited. The group of Ad-Rb94 combined with irradiation resulted in greater inhibition of cells growth, reached 45.49 % , compared with Ad- Rb94 infection (20.26 % ) and radiation group (35.11 % ) (χ~2 = 14.25,P <0.05). Cells of G_1 phases and apoptosis of K150 cells for the Ad-Rb94 combined with irradiation group were the highest, reached 73.4% and 15.7% , respectively. The combination of Ad-Rb94 and irradiation resulted in the greatest expression of retinoblastoma 94 gene, which was 1.87 fold and 2.92 fold of Ad-Rb94 infection and irradiation, respectively. Conclusions Ad-Rb94 gene transfection combined with irradiation shows the synergism for the inhibition of esophageal cancer K150 cells growth.