CAJ | 학술논문

探讨Wistar大鼠脑微血管周细胞（brain microvascular pericyte，BMP）和脊髓微血管周细胞（spinal cord microvascular pericyte，SCMP）之间的差异。运用超高速离心法获取脑和脊髓微血管，再用周细胞培养基培养，使周细胞爬出，然后用NG2和PDGFRβ鉴定周细胞，用鬼笔环肽着染F-actin，用流式细胞仪测定细胞周期，用免疫印记分析实验测定周细胞功能蛋白。结果表明：两种周细胞的形态有明显差异，BMP表达的F-actin显著多于SCMP，两种周细胞的细胞周期无显著差异，BMP相比于SCMP表达更多量的α-SMA、NG2和PDGFRβ。更多的了解两种周细胞的异同点，为研究其在中枢神经系统生理和病理状态下的作用奠定基础。
탐토Wistar대서뇌미혈관주세포（brain microvascular pericyte，BMP）화척수미혈관주세포（spinal cord microvascular pericyte，SCMP）지간적차이。운용초고속리심법획취뇌화척수미혈관，재용주세포배양기배양，사주세포파출，연후용NG2화PDGFRβ감정주세포，용귀필배태착염F-actin，용류식세포의측정세포주기，용면역인기분석실험측정주세포공능단백。결과표명：량충주세포적형태유명현차이，BMP표체적F-actin현저다우SCMP，량충주세포적세포주기무현저차이，BMP상비우SCMP표체경다량적α-SMA、NG2화PDGFRβ。경다적료해량충주세포적이동점，위연구기재중추신경계통생리화병리상태하적작용전정기출。
To compare the different characteristics of brain microvascular pericytes (BMP)and spinal cord microvacularpericytes (SCMP),they were isolated,cultured and identified.High-speed centrifugal method was used to obtain the microvassels from brain and spinal cord.Then the microvessels were cultivated in the pericyte-specific medium.Pericyte was isolated from the microvessels. NG2 and PDGFRβ were used to identify the pericytes.FITC-labeled phalloidin was used to stain F-actin.The cell cycles of BMP and SCMP were detected by flow cytometry.Western blot assay was applied to analyze the functional proteins of pericytes.The results indicated that BMPs possessed bigger cell nucleus and more unfolded cytoplasm than SCMPs.BMPs expressed much more F-actin than SCMPs (P<0.01).There was no significant difference between the two types of pericytes in cell cycle.BMP expressed more α-SMA、NG2 and PDGFRβthan SCMPs.These distinguishing features may reflect the more widespread differences between the BBB and BSCB which directly impact pathophysiological processes in various major diseases.