CAJ | 학술논문

目的：观察犬肺静脉的纤维走行方向及起搏细胞的存在，研究犬急性心房颤动模型时肺静脉及左心房中起搏电流（funny current ，If）通道亚单位超极化激活的环核苷酸门控通道（hyperpolarization-activated cyclic nucleotide-gated cation channel ， HCN）1、2、4的表达变化。方法14只健康杂种犬随机分为房颤组与对照组，快速心房起搏建立犬急性房颤模型，取对照组的肺静脉肌袖组织做苏木素-伊红（HE）及高碘酸-希夫反应（PAS）染色；取两组的肺静脉、左心房组织，用逆转录-PCR（RT-PCR）方法测定HCN1、HCN2、HCN43种通道的mRNA表达水平。结果肺静脉尤其是肺静脉左心房交界处肌纤维排列紊乱；肺静脉肌袖的内膜面可以见到传导细胞；两组犬的肺静脉及左心房中均有 HCN2、HCN4的mRNA表达，而无 HCN1的表达。 HCN4的mRNA表达水平从高到低依次为：房颤组肺静脉、房颤组左心房、对照组肺静脉、对照组左心房，组间比较差异具有统计学意义（P＜0．05）。HCN2的mRNA表达水平从高到低依次为：房颤组肺静脉、房颤组左心房、对照组，组间比较差异具有统计学意义（P＜0．05），对照组的肺静脉与对照组左心房中HCN2的mRNA表达水平无明显差异。结论犬肺静脉肌袖存在纤维各向异性及传导细胞，急性房颤时肺静脉及左心房 HCN2、HCN4的表达上调，可能参与房颤的发生与维持。
목적：관찰견폐정맥적섬유주행방향급기박세포적존재，연구견급성심방전동모형시폐정맥급좌심방중기박전류（funny current ，If）통도아단위초겁화격활적배핵감산문공통도（hyperpolarization-activated cyclic nucleotide-gated cation channel ， HCN）1、2、4적표체변화。방법14지건강잡충견수궤분위방전조여대조조，쾌속심방기박건립견급성방전모형，취대조조적폐정맥기수조직주소목소-이홍（HE）급고전산-희부반응（PAS）염색；취량조적폐정맥、좌심방조직，용역전록-PCR（RT-PCR）방법측정HCN1、HCN2、HCN43충통도적mRNA표체수평。결과폐정맥우기시폐정맥좌심방교계처기섬유배렬문란；폐정맥기수적내막면가이견도전도세포；량조견적폐정맥급좌심방중균유 HCN2、HCN4적mRNA표체，이무 HCN1적표체。 HCN4적mRNA표체수평종고도저의차위：방전조폐정맥、방전조좌심방、대조조폐정맥、대조조좌심방，조간비교차이구유통계학의의（P＜0．05）。HCN2적mRNA표체수평종고도저의차위：방전조폐정맥、방전조좌심방、대조조，조간비교차이구유통계학의의（P＜0．05），대조조적폐정맥여대조조좌심방중HCN2적mRNA표체수평무명현차이。결론견폐정맥기수존재섬유각향이성급전도세포，급성방전시폐정맥급좌심방 HCN2、HCN4적표체상조，가능삼여방전적발생여유지。
Objective To observe the anisotropy of myocardial fiber orientation and conduction cells in canine pulmonary vein , and to investigate the expression of funny current (If ) channel subunit HCN1 ,HCN2 ,HCN4 mRNA in cadiocytes of pulmonary veins and left atrial in acute atrial fibrillation (AF) modle of caine .Methods Fourteen adult mongrel canines were randomly divided into atrial fibrillation group(n=7) and control group(n=7) .A model of acute AF has been developed in rapid atrial pacing (RAP) . The cardiocytes in control group were detect using hematoxylin hematoxylin and eosin (HE) and Periodic acid-Schiff(PAS) stain in the pulmonary vein .The mRNA expressions of HCN1 ,HCN2 ,HCN4 in two group were quantified by semiquantitative reverse transcription transcription-polymerase chain reaction(RT-PCR) .Results There were complex myocardial fiber orientation in pul-monary veins especially in PV-LA junction .PAS-positive cells could be seen in the endomembrane of pulmonary veins muscle sleeve .There were mRNA expression of HCN2 and HCN4 in pulmonary veins and left arial in two group ,but there was no mRNA expression of HCN1 .The mRNA levels of HCN4 from high to low were:pulmonary veins of atrial fibrillation group ,left atrial of atrial fibrillation group ,pulmonary veins of control group ,left atrial of control group(P<0 .05) .HCN2 mRNA levels from high to low were:pulmonary veins of atrial fibrillation group ,left atrial of atrial fibrillation group ,control group(P<0 .05) .There were no difference in the HCN2 mRNA level in pulmonary veins and left atrial in control group (P>0 .05) .Conclusion There are anisotro-py ,conduction cells in pulmonary veins .Up-regulation of pulmonary veins and left atrial HCN 2 ,HCN4 mRNA in atrial fibrillation caine ,may be correlated with the atrial fibrillation and maintenance .