CAJ | 학술논문

为建立VBNC状态沙门氏菌实验室快速诱导条件，以4℃低温诱导为对照组，在此基础上，添加不同浓度Cu2+和冰醋酸作为快速诱导条件进行试验。结果表明，在4℃低温条件下，添加2～4 mM Cu2+对沙门氏菌进入VBNC状态有明显促进作用，与传统诱导条件相比，诱导时间平均提前15 d。对诱导进入VBNC状态的沙门氏菌进行16S rRNA鉴定，结果证明，所得菌为VBNC状态沙门氏菌。
위건립VBNC상태사문씨균실험실쾌속유도조건，이4℃저온유도위대조조，재차기출상，첨가불동농도Cu2+화빙작산작위쾌속유도조건진행시험。결과표명，재4℃저온조건하，첨가2～4 mM Cu2+대사문씨균진입VBNC상태유명현촉진작용，여전통유도조건상비，유도시간평균제전15 d。대유도진입VBNC상태적사문씨균진행16S rRNA감정，결과증명，소득균위VBNC상태사문씨균。
In order to establish the rapid induction condition of VBNC state Salmonellapulorum, the induction at the low temperature of 4℃was selected as the control group, and on this basis, the experiment was conducted through adding different concentrations of Cu2+ and glacial acetic acid as the rapid induction condition. The results show that adding 2~4 mM Cu2+ could obviously promoteSalmonella into VBNC state at 4℃ low temperature, and compared with the traditional induction condition, the induction time could be advanced 15 d averagely. TheSalmonella entering into the VBNC state was identified by 16S rRNA test, and the results proved that the stains wereSalmonella of VBNC state.